PXD028288

PXD028288 is an original dataset announced via ProteomeXchange.

Title	LC-MSMS with isobaric tagging of Pgrmc1 KO mouse liver membranes expressing Flag-Pgrmc1 or Y113F Flag-Pgrmc1
Description	Progesterone receptor membrane component 1 (PGRMC1) is a heme binding protein implicated in a wide range of cellular functions. Our previous studies showed that PGRMC1 binds to cytochromes P450 in yeast and mammalian cells and promotes activity of these enzymes. Recently, the paralog PGRMC2 was shown to function as an intracellular heme chaperone. Here, we examined the function of the Pgrmc1 Y113F mutant that is defective for heme iron coordination by measuring levels of membrane proteins in livers from Pgrmc1 knockout mice infected with AAV8 expressing either GFP, Flag-Pgrmc1, or Y113F Flag-Pgrmc1. These experiments (1) confirmed findings in uninfected wild-type and Pgrmc1 knockout mice and (2) revealed that Pgrmc1 Y113F functions to stabilize cytochrome P450 enzymes similar to wild-type Pgrmc1.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_12:54:22.088.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Espenshade
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:10:56	ID requested
⏵ 1	2022-02-16 12:54:22	announced

McGuire MR, Mukhopadhyay D, Myers SL, Mosher EP, Brookheart RT, Kammers K, Sehgal A, Selen ES, Wolfgang MJ, Bumpus NN, Espenshade PJ, Progesterone receptor membrane component 1 (PGRMC1) binds and stabilizes cytochromes P450 through a heme-independent mechanism. J Biol Chem, 297(5):101316(2021) [pubmed]

submitter keyword: Mouse, Liver, Membrane, LC-MSMS, TMT, isobaric tag, Pgrmc1, Flag-Pgrmc1, Y113F Flag-Pgrmc1

Peter J. Espenshade
contact affiliation	Department of Cell Biology School of Medicine Johns Hopkins University Baltmore, MD USA
contact email	peter.espenshade@jhmi.edu
lab head
Peter Espenshade
contact affiliation	Johns Hopkins University
contact email	peter.espenshade@jhmi.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD028288

PRIDE project URI

• PRIDE
  1. PXD028288
     1. Label: PRIDE project
     2. Name: LC-MSMS with isobaric tagging of Pgrmc1 KO mouse liver membranes expressing Flag-Pgrmc1 or Y113F Flag-Pgrmc1