PXD028284

PXD028284 is an original dataset announced via ProteomeXchange.

Title	LC-MSMS with isobaric tagging on Flag-coimmunoprecipitation eluates from Flag-Pgrmc1 and Y113F Flag-Pgrmc1 expressing Pgrmc1 KO mouse liver membranes
Description	Progesterone receptor membrane component 1 (PGRMC1) is a heme binding protein implicated in a wide range of cellular functions. Our previous studies showed that PGRMC1 binds to cytochromes P450 in yeast and mammalian cells and promotes activity of these enzymes. Recently, the paralog PGRMC2 was shown to function as an intracellular heme chaperone. Here, we examined the function of the Pgrmc1 by identifying binding partners for wild-type Pgrmc1 and the Pgrmc1 Y113F mutant that is defective for heme iron coordination. Pgrmc1 knockout mice were infected with AAV8 expressing either GFP, Flag-Pgrmc1, or Y113F Flag-Pgrmc1, and Flag-Pgrmc1 was affinity purified using anti-Flag antibodies from extracts of liver membranes. These experiments (1) demonstrated that Pgrmc1 binds to different cytochrome P450 enzymes and (2) revealed that Pgrmc1 Y113F specifically fails to bind to ferrochelatase.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_13:12:11.553.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Espenshade
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:10:42	ID requested
⏵ 1	2022-02-15 13:12:12	announced

McGuire MR, Mukhopadhyay D, Myers SL, Mosher EP, Brookheart RT, Kammers K, Sehgal A, Selen ES, Wolfgang MJ, Bumpus NN, Espenshade PJ, Progesterone receptor membrane component 1 (PGRMC1) binds and stabilizes cytochromes P450 through a heme-independent mechanism. J Biol Chem, 297(5):101316(2021) [pubmed]

submitter keyword: Mouse, Liver, Membrane, LC-MSMS, TMT, isobaric tag, Pgrmc1, Flag-Pgrmc1, Y113F Flag-Pgrmc1, co-immunoprecipitation, eluate, bound

Peter J. Espenshade
contact affiliation	Department of Cell Biology, School of Medicine, Johns Hopkins University, Baltimore, MD, USA
contact email	peter.espenshade@jhmi.edu
lab head
Peter Espenshade
contact affiliation	Johns Hopkins University
contact email	peter.espenshade@jhmi.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028284
     1. Label: PRIDE project
     2. Name: LC-MSMS with isobaric tagging on Flag-coimmunoprecipitation eluates from Flag-Pgrmc1 and Y113F Flag-Pgrmc1 expressing Pgrmc1 KO mouse liver membranes