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PXD028284

PXD028284 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLC-MSMS with isobaric tagging on Flag-coimmunoprecipitation eluates from Flag-Pgrmc1 and Y113F Flag-Pgrmc1 expressing Pgrmc1 KO mouse liver membranes
DescriptionProgesterone receptor membrane component 1 (PGRMC1) is a heme binding protein implicated in a wide range of cellular functions. Our previous studies showed that PGRMC1 binds to cytochromes P450 in yeast and mammalian cells and promotes activity of these enzymes. Recently, the paralog PGRMC2 was shown to function as an intracellular heme chaperone. Here, we examined the function of the Pgrmc1 by identifying binding partners for wild-type Pgrmc1 and the Pgrmc1 Y113F mutant that is defective for heme iron coordination. Pgrmc1 knockout mice were infected with AAV8 expressing either GFP, Flag-Pgrmc1, or Y113F Flag-Pgrmc1, and Flag-Pgrmc1 was affinity purified using anti-Flag antibodies from extracts of liver membranes. These experiments (1) demonstrated that Pgrmc1 binds to different cytochrome P450 enzymes and (2) revealed that Pgrmc1 Y113F specifically fails to bind to ferrochelatase.
HostingRepositoryPRIDE
AnnounceDate2022-02-15
AnnouncementXMLSubmission_2022-02-15_13:12:11.553.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPeter Espenshade
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-09 08:10:42ID requested
12022-02-15 13:12:12announced
Publication List
McGuire MR, Mukhopadhyay D, Myers SL, Mosher EP, Brookheart RT, Kammers K, Sehgal A, Selen ES, Wolfgang MJ, Bumpus NN, Espenshade PJ, Progesterone receptor membrane component 1 (PGRMC1) binds and stabilizes cytochromes P450 through a heme-independent mechanism. J Biol Chem, 297(5):101316(2021) [pubmed]
Keyword List
submitter keyword: Mouse, Liver, Membrane, LC-MSMS, TMT, isobaric tag, Pgrmc1, Flag-Pgrmc1, Y113F Flag-Pgrmc1, co-immunoprecipitation, eluate, bound
Contact List
Peter J. Espenshade
contact affiliationDepartment of Cell Biology, School of Medicine, Johns Hopkins University, Baltimore, MD, USA
contact emailpeter.espenshade@jhmi.edu
lab head
Peter Espenshade
contact affiliationJohns Hopkins University
contact emailpeter.espenshade@jhmi.edu
dataset submitter
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Dataset FTP location
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