PXD028284 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | LC-MSMS with isobaric tagging on Flag-coimmunoprecipitation eluates from Flag-Pgrmc1 and Y113F Flag-Pgrmc1 expressing Pgrmc1 KO mouse liver membranes |
Description | Progesterone receptor membrane component 1 (PGRMC1) is a heme binding protein implicated in a wide range of cellular functions. Our previous studies showed that PGRMC1 binds to cytochromes P450 in yeast and mammalian cells and promotes activity of these enzymes. Recently, the paralog PGRMC2 was shown to function as an intracellular heme chaperone. Here, we examined the function of the Pgrmc1 by identifying binding partners for wild-type Pgrmc1 and the Pgrmc1 Y113F mutant that is defective for heme iron coordination. Pgrmc1 knockout mice were infected with AAV8 expressing either GFP, Flag-Pgrmc1, or Y113F Flag-Pgrmc1, and Flag-Pgrmc1 was affinity purified using anti-Flag antibodies from extracts of liver membranes. These experiments (1) demonstrated that Pgrmc1 binds to different cytochrome P450 enzymes and (2) revealed that Pgrmc1 Y113F specifically fails to bind to ferrochelatase. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-15 |
AnnouncementXML | Submission_2022-02-15_13:12:11.553.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Peter Espenshade |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-09-09 08:10:42 | ID requested | |
⏵ 1 | 2022-02-15 13:12:12 | announced | |
Publication List
McGuire MR, Mukhopadhyay D, Myers SL, Mosher EP, Brookheart RT, Kammers K, Sehgal A, Selen ES, Wolfgang MJ, Bumpus NN, Espenshade PJ, Progesterone receptor membrane component 1 (PGRMC1) binds and stabilizes cytochromes P450 through a heme-independent mechanism. J Biol Chem, 297(5):101316(2021) [pubmed] |
Keyword List
submitter keyword: Mouse, Liver, Membrane, LC-MSMS, TMT, isobaric tag, Pgrmc1, Flag-Pgrmc1, Y113F Flag-Pgrmc1, co-immunoprecipitation, eluate, bound |
Contact List
Peter J. Espenshade |
contact affiliation | Department of Cell Biology, School of Medicine, Johns Hopkins University, Baltimore, MD, USA |
contact email | peter.espenshade@jhmi.edu |
lab head | |
Peter Espenshade |
contact affiliation | Johns Hopkins University |
contact email | peter.espenshade@jhmi.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028284
- Label: PRIDE project
- Name: LC-MSMS with isobaric tagging on Flag-coimmunoprecipitation eluates from Flag-Pgrmc1 and Y113F Flag-Pgrmc1 expressing Pgrmc1 KO mouse liver membranes