PXD028282

PXD028282 is an original dataset announced via ProteomeXchange.

Title	Microdissected pyramidal cell proteomics of Alzheimer brain reveals alterations in Creatine Kinase B, 14-3-3-g and Heat Shock Cognate 71
Description	Novel insights on proteins involved in Alzheimer disease (AD) are needed. Since multiple cell types and matrix components are altered in AD, bulk analysis of brain tissue may be difficult to interpret. In the current study, we isolated pyramidal cells from the CA1 region of the hippocampus from five AD and five neurologically healthy donors using laser capture microdissection. The samples were analysed by proteomics using 18O labeled internal standard and nano-HPLC MS/MS for relative quantification. Fold change between AD and control was calculated for the proteins that were identified in at least two individual proteomes from each group. From the ten cases analyzed, sixty-two proteins were identified in at least two AD cases and two control cases. Creatine Kinase B-type (CKB), 14-3-3-g and Heat Shock Cognate 71 (Hsc71) which have not been extensively studied in the context of human AD brain previously, were selected for further studies by immunohistochemistry (IHC). In hippocampus, semi-quantitative measures of IHC staining of the three proteins confirmed the findings from our proteomic analysis. Studies of the same proteins in frontal cortex revealed that the alterations remained for CKB and 14-3-3-g but not for Hsc71. Protein upregulation in CA1 neurons of final stage AD is either a result from detrimental, pathological effects, or from cell specific protective response mechanisms in surviving neurons. Based on previous findings from experimental studies, CKB and Hsc71 likely exhibit protective effects, whereas 14-3-3-g represents a detrimental path. These new players could represent pathways of importance for development of new therapeutic strategies.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_13:10:58.265.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anna Matton
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	(18)O label at both C-terminal oxygens; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:10:27	ID requested
⏵ 1	2022-02-15 13:10:59	announced

Sandebring-Matton A, Axenhus M, Bogdanovic N, Winblad B, Schedin-Weiss S, Nilsson P, Tjernberg LO, , and Heat Shock Cognate 71. Front Aging Neurosci, 13():735334(2021) [pubmed]

submitter keyword: Alzheimer disease, homo sapiens, Q Exactive, O18 labelling, quantitative MS

Lars Tjernberg
contact affiliation	Karolinska Institutet
contact email	lars.tjernberg@ki.se
lab head
Anna Matton
contact affiliation	PhD
contact email	anna.matton@ki.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028282
     1. Label: PRIDE project
     2. Name: Microdissected pyramidal cell proteomics of Alzheimer brain reveals alterations in Creatine Kinase B, 14-3-3-g and Heat Shock Cognate 71