PXD028244

PXD028244 is an original dataset announced via ProteomeXchange.

Title	A bioorthogonal chemical reporter for fatty acid synthase-dependent protein acylation
Description	Mammalian fatty acid synthase (FASN) is a lipogenic enzyme that catalyzes the formation of the long chain saturated fatty acid palmitate from acetyl and malonyl CoA in the presence of NADPH. Mammalian cells acquire fatty acids through dietary sources or through FASN. Although most mammalian cells express FASN at low levels, it is upregulated in cancers and during replication of many viruses. The precise role of FASN in disease pathogenesis is poorly understood, and whether de novo fatty acid synthesis contributes to host or viral protein acylation has been traditionally difficult to study. We describe a cell permeable, click-chemistry compatible alkynyl-acetate analog (5-Hexynoic acid, or "Alk-4") that functions as a reporter of FASN-dependent protein acylation. Alk-4 metabolic labeling enabled biotin-based purification and identification of more than 200 FASN-dependent acylated cellular proteins. Alk-4 also labeled the palmitoylated host protein IFITM3 (Interferon inducible transmembrane protein-3), a restriction factor for Influenza, and the myristoylated HIV-1 MA (Matrix) protein. Thus, Alk-4 is a useful bioorthogonal tool to selectively probe FASN-mediated protein acylation in normal and diseased states.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_04:01:55.404.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Krithika Karthigeyan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 07:57:05	ID requested
⏵ 1	2022-02-16 04:01:56	announced

Karthigeyan KP, Zhang L, Loiselle DR, Haystead TAJ, Bhat M, Yount JS, Kwiek JJ, A bioorthogonal chemical reporter for fatty acid synthase-dependent protein acylation. J Biol Chem, 297(5):101272(2021) [pubmed]

submitter keyword: protein acylation, fatty acid synthase, click chemistry, S-palmitoylation, N-myristoylation,

Jesse John Kwiek
contact affiliation	Department of Microbiology, The Ohio State University
contact email	kwiek.2@osu.edu
lab head
Krithika Karthigeyan
contact affiliation	The Ohio State University
contact email	karthigeyan.1@osu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028244
     1. Label: PRIDE project
     2. Name: A bioorthogonal chemical reporter for fatty acid synthase-dependent protein acylation