PXD028242

PXD028242 is an original dataset announced via ProteomeXchange.

Title	Relative Quantification of the Effect of Blood Meal Iron on Protein Expression in Mosquito Ovaries 24 h Post Feeding
Description	Aedes aegypti [Linnaeus in Hasselquist (Diptera: Culicidae); yellow fever mosquito] transmits several viruses that infect millions of people each year including, Zika, dengue, yellow fever, chikungunya, and West Nile. Disease transmission occurs during blood feeding. Only the females blood feed as they require a bloodmeal for oogenesis. In the bloodmeal, females receive a substantial iron load in the forms of holo-transferrin and hemoglobin. We are interested in the effects of the iron in a bloodmeal on the expression of proteins during oogenesis. Our previous data showed that during digestion of a blood meal, the gut iron concentration decreases 10-fold, while that of the ovaries doubles from ingestion to 72 hours post feeding. Approximately 72 hours post feeding, eggs are laid with ~125 ng Fe each. We are interested in the effects of the blood meal iron on the expression of proteins detected in the ovaries during the early oogenesis, 24 hours post feeding, before eggs are laid. We have used tandem mass tag-labeling proteomics to quantify proteins expressed at this early stage following feeding of a controlled iron diet. Our findings provide the first quantitative report of differential ovary protein expression in early oogenesis in mosquitoes fed three different iron diets.
HostingRepository	PRIDE
AnnounceDate	2022-03-26
AnnouncementXML	Submission_2022-03-25_17:15:06.786.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028242
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dawn Geiser
SpeciesList	scientific name: Aedes aegypti; NCBI TaxID: 7159;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 07:56:53	ID requested
⏵ 1	2022-03-25 17:15:07	announced

Geiser DL, Li W, Pham DQ, Wysocki VH, Winzerling JJ, Shotgun and TMT-Labeled Proteomic Analysis of the Ovarian Proteins of an Insect Vector, Aedes aegypti (Diptera: Culicidae). J Insect Sci, 22(2):(2022) [pubmed]

submitter keyword: Aedes aegypti, bloodmeal, iron, ovary, proteomics

Joy J. Winzerling
contact affiliation	Nutritional Sciences, Division of Agriculture, Life and Veterinary Sciences, University of Arizona, Tucson, Arizona 85721, USA
contact email	jwinzerl@ag.arizona.edu
lab head
Dawn Geiser
contact affiliation	University of Arizona
contact email	dlgeiser@email.arizona.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028242
     1. Label: PRIDE project
     2. Name: Relative Quantification of the Effect of Blood Meal Iron on Protein Expression in Mosquito Ovaries 24 h Post Feeding