PXD028239

PXD028239 is an original dataset announced via ProteomeXchange.

Title	Inherited defect in ST6GalNAc1 reveals roles of sialylation in intestinal homeostasis
Description	Chronic inflammatory bowel disease (IBD) causes disability, suffering and risk of colon cancer in over 30 million people globally. Here, we investigate 3 kindreds of IBD with mutations in the N-acetylgalactosamide alpha-2,6-sialyltransferase 1/ST6GALNAC1 (ST6) glycosyltransferase gene that is uniquely expressed in the goblet cells (GCs). These mutations cause defective sialic acid (SA) conjugation, elimination of the onco-antigen S-Tn (Sialyl-Tn), and altered glycosylation of the MUC2 protein, a key component of intestinal mucus. Decreased MUC2 sialylation increases susceptibility to bacterial proteolytic degradation and compromises the gastrointestinal (GI) mucus barrier. Mice harboring the patient ST6 mutations recapitulate human colitis and reveal that defective sialylation causes dysbiosis, butyrate overproduction, and impaired GI stem cell proliferation during injury. Thus, this new genetic disease illustrates how sialylation controls host-microbe homeostasis and reveals several new potential approaches to IBD treatment.
HostingRepository	PRIDE
AnnounceDate	2022-03-17
AnnouncementXML	Submission_2022-03-17_09:53:22.488.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanlong Ji
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	complex glycosylation
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 07:56:38	ID requested
⏵ 1	2022-03-17 09:53:22	announced

Dataset with its publication pending

submitter keyword: Mus musculus (mouse), Homo sapiens (human), Orbitrap Fusion Lumos

Kuan-Ting Pan
contact affiliation	Clinical Proteomics, Frankfurt Cancer Institute; Haematology/Oncology, Department of Medicine II Goethe University Hospital Frankfurt
contact email	Kuan-Ting.Pan@kgu.de
lab head
Yanlong Ji
contact affiliation	Max-Planck-Institute for Biophysical Chemistry
contact email	yanlong.ji@mpibpc.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028239
     1. Label: PRIDE project
     2. Name: Inherited defect in ST6GalNAc1 reveals roles of sialylation in intestinal homeostasis