PXD028238

PXD028238 is an original dataset announced via ProteomeXchange.

Title	Wild-type and Pgrmc1 KO mouse liver membrane LC-MSMS with isobaric tagging
Description	Progesterone receptor membrane component 1 (PGRMC1) is a heme binding protein implicated in a wide range of cellular functions. Our previous studies showed that PGRMC1 binds to cytochromes P450 in yeast and mammalian cells and promotes activity of these enzymes. Recently, the paralog PGRMC2 was shown to function as an intracellular heme chaperone. Here, we examined the function of Pgrmc1 in mouse liver by measuring levels of membrane proteins in livers from wild-type and Pgrmc1 knockout mice. These experiments revealed that Pgrmc1 knockout mouse livers contained reduced levels of cytochrome P450 enzymes.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_09:51:06.823.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Espenshade
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; methylthiolated residue; monohydroxylated residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 07:56:36	ID requested
⏵ 1	2022-02-16 09:51:07	announced

McGuire MR, Mukhopadhyay D, Myers SL, Mosher EP, Brookheart RT, Kammers K, Sehgal A, Selen ES, Wolfgang MJ, Bumpus NN, Espenshade PJ, Progesterone receptor membrane component 1 (PGRMC1) binds and stabilizes cytochromes P450 through a heme-independent mechanism. J Biol Chem, 297(5):101316(2021) [pubmed]

submitter keyword: Mouse, Liver, Membrane, LC-MSMS, TMT, isobaric tag, Pgrmc1

Peter J. Espenshade
contact affiliation	Department of Cell Biology, School of Medicine, Johns Hopkins University, Baltimore, MD, USA
contact email	peter.espenshade@jhmi.edu
lab head
Peter Espenshade
contact affiliation	Johns Hopkins University
contact email	peter.espenshade@jhmi.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD028238

PRIDE project URI

• PRIDE
  1. PXD028238
     1. Label: PRIDE project
     2. Name: Wild-type and Pgrmc1 KO mouse liver membrane LC-MSMS with isobaric tagging