PXD028231

PXD028231 is an original dataset announced via ProteomeXchange.

Title	AK2 promotes the migration and invasion of lung adenocarcinoma by activating TGF-β/Smad pathway in vitro and in vivo
Description	Adenylate kinase 2 (AK2) is a wide-spread and highly conserved protein kinase whose main function is to catalyze the exchange of nucleotide phosphate groups. In this study, we showed that AK2 regulated tumor cell metastasis in lung adenocarcinoma. Positive expression of AK2 is related to lung adenocarcinoma progression and poor survival of patients. Knockdown or knockout of AK2 inhibited, while overexpression of AK2 promoted, human lung adenocarcinoma cell migration and invasion ability. Differential proteomics results showed that AK2 might be closely related to epithelial-mesenchymal transition (EMT). Further research indicated that AK2 regulated EMT occurrence through the Smad-dependent classical signaling pathways as measured by western blot and qPCR assays. Additionally, in vivo experiments showed that AK2-knockout in human lung tumor cells reduced their EMT-like features and formed fewer metastatic nodules both in liver and in lung tissues. In conclusion, we uncover a cancer metastasis-promoting role for AK2 and provide a rationale for targeting AK2 as a potential therapeutic approach for lung cancer.
HostingRepository	iProX
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2023-12-21_20:33:14.640.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fangfang Cai
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-08 20:36:26	ID requested
1	2021-09-15 19:57:59	announced
⏵ 2	2023-12-21 20:33:15	announced	2023-12-22: Update publication information.

Cai F, Xu H, Zha D, Wang X, Li P, Yu S, Yao Y, Chang X, Chen J, Lu Y, Hua ZC, Zhuang H, . Front Pharmacol, 12():714365(2021) [pubmed]

submitter keyword: AK2, lung adenocarcinoma, EMT, tumor metastasis, TGF-β

Hongqin Zhuang
contact affiliation	Nanjing University
contact email	hqzhuang@nju.edu.cn
lab head
Fangfang Cai
contact affiliation	Nanjing University
contact email	1980160925@qq.com
dataset submitter

iProX dataset URI