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PXD028218

PXD028218 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe obligate intracellular bacterium Orientia tsutsugamushi differentiates into a developmentally distinct extracellular state
DescriptionOrientia tsutsugamushi (Ot) is an obligate intracellular bacterium in the family Rickettsiaceae that causes scrub typhus, a severe mite-borne human disease. Ot is unusual amongst Rickettsiaceae in budding out of infected cells in a process resembling that used by enveloped viruses. Here, we show that Ot bacteria that have budded out of host cells are in a distinct developmental stage compared with intracellular bacteria. We refer to these two stages as intracellular and extracellular bacteria (IB and EB, respectively). These two forms differ in physical properties: IB is elongated, and EB is round. Additionally, IB has higher levels of peptidoglycan and is physically robust compared with EB. The two bacterial forms differentially express proteins involved in bacterial physiology and host-pathogen interactions, specifically those involved in bacterial dormancy and stress response, secreted bacterial effectors, and outer membrane autotransporter proteins ScaA and ScaC. Whilst both populations are infectious, entry of IB Ot is sensitive to inhibitors of both clathrin-mediated endocytosis and micropinocytosis, whereas entry of EB Ot is only sensitive to a micropinocytosis inhibitor. Our identification and detailed characterization of two developmental forms of Ot significantly advances our understanding of the intracellular lifecycle of an important human pathogen.
HostingRepositoryjPOST
AnnounceDate2022-05-11
AnnouncementXMLSubmission_2023-04-02_20:19:49.820.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRadoslaw Sobota
SpeciesList scientific name: Orientia tsutsugamushi; NCBI TaxID: 784;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue; deamidated L-glutamine; deamidated L-asparagine
Instrumentinstrument
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-06 22:41:16ID requested
12022-05-10 20:17:13announced
22022-09-18 03:07:26announced2022-09-18: Updated FTP location.
32023-04-02 20:19:50announced2023-04-03: Updated PubMed.
Publication List
Atwal S, Wongsantichon J, Giengkam S, Saharat K, Pittayasathornthun YJ, Chuenklin S, Wang LC, Chung T, Huh H, Lee SH, Sobota RM, Salje J, The obligate intracellular bacterium Orientia tsutsugamushi differentiates into a developmentally distinct extracellular state. Nat Commun, 13(1):3603(2022) [pubmed]
Keyword List
submitter keyword: Orientia tsutsugamushi, scrub typhus
Contact List
Jeanne Salje
lab head
Radoslaw Sobota
contact affiliationIMCB A-STAR Singapore
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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