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PXD028209

PXD028209 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHDL quality feature revealed by proteome-lipidome connectivity are associated with atherosclerotic disease
DescriptionLipoprotein, especially high density lipoprotein (HDL) particles are composed of multiple heterogeneous subgroups containing various proteins and lipids. The molecular distribution among these subgroups is closely related to cardiovascular disease (CVD). Here, we established high-resolution proteomics and lipidomics (HiPL) methods to depict the molecular profiles across lipoprotein (Lipo-HiPL) and HDL (HDL-HiPL) subgroups by optimizing the resolution of anion-exchange chromatography (AEC) and comprehensive quantification of proteins and lipids on omics level. Furthermore, based on the Pearson correlation coefficient (PCC) analysis of molecular profiles across high-resolution subgroups, we achieved the relationship of proteome-lipidome connectivity (PLC) for lipoprotein and HDL particles. By application of these methods to high fat high cholesterol (HFHC) diet rabbits and acute coronary syndrome (ACS) patients, we uncovered the delicate dynamics of molecular profile and reconstruction of lipoprotein and HDL particles. Of note, the PLC features revealed by the HDL-HiPL method discriminated ACS from healthy individuals better than direct proteome and lipidome quantification, or PLC features revealed by the Lipo-HiPL method, suggesting their potential in ACS diagnosis. Together, we established HiPL methods to trace the dynamics of molecular profile and PLC of lipoprotein and even HDL during the development of CVD.
HostingRepositoryiProX
AnnounceDate2021-09-03
AnnouncementXMLSubmission_2023-02-02_19:08:50.578.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDandan Wang
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Oryctolagus cuniculus; NCBI TaxID: 9986;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-05 19:50:08ID requested
12021-11-29 19:44:21announced
22023-02-02 19:08:51announced2023-02-03: Update publication information.
Publication List
Wang D, Yu B, Li Q, Guo Y, Koike T, Koike Y, Wu Q, Zhang J, Mao L, Tang X, Sun L, Lin X, Wu J, Chen YE, Peng D, Zeng R, lipidome connectivity are associated with atherosclerotic disease. J Mol Cell Biol, 14(3):(2022) [pubmed]
Keyword List
submitter keyword: HDL, high-resolution, proteomics, lipidomics, atherosclerotic disease
Contact List
Rong Zeng
contact affiliationShanghai Institute of Biochemistry and Cell Biology, CAS Center for Excellence in Molecular Cell Science
contact emailzr@sibcb.ac.cn
lab head
Dandan Wang
contact affiliationShanghai Institute of Biochemistry and Cell Biology, CAS Center for Excellence in Molecular Cell Science
contact emailwangdandan2015@sibcb.ac.cn
dataset submitter
Full Dataset Link List
iProX dataset URI