PXD028209

PXD028209 is an original dataset announced via ProteomeXchange.

Title	HDL quality feature revealed by proteome-lipidome connectivity are associated with atherosclerotic disease
Description	Lipoprotein, especially high density lipoprotein (HDL) particles are composed of multiple heterogeneous subgroups containing various proteins and lipids. The molecular distribution among these subgroups is closely related to cardiovascular disease (CVD). Here, we established high-resolution proteomics and lipidomics (HiPL) methods to depict the molecular profiles across lipoprotein (Lipo-HiPL) and HDL (HDL-HiPL) subgroups by optimizing the resolution of anion-exchange chromatography (AEC) and comprehensive quantification of proteins and lipids on omics level. Furthermore, based on the Pearson correlation coefficient (PCC) analysis of molecular profiles across high-resolution subgroups, we achieved the relationship of proteome-lipidome connectivity (PLC) for lipoprotein and HDL particles. By application of these methods to high fat high cholesterol (HFHC) diet rabbits and acute coronary syndrome (ACS) patients, we uncovered the delicate dynamics of molecular profile and reconstruction of lipoprotein and HDL particles. Of note, the PLC features revealed by the HDL-HiPL method discriminated ACS from healthy individuals better than direct proteome and lipidome quantification, or PLC features revealed by the Lipo-HiPL method, suggesting their potential in ACS diagnosis. Together, we established HiPL methods to trace the dynamics of molecular profile and PLC of lipoprotein and even HDL during the development of CVD.
HostingRepository	iProX
AnnounceDate	2021-09-03
AnnouncementXML	Submission_2023-02-02_19:08:50.578.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dandan Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Oryctolagus cuniculus; NCBI TaxID: 9986;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-05 19:50:08	ID requested
1	2021-11-29 19:44:21	announced
⏵ 2	2023-02-02 19:08:51	announced	2023-02-03: Update publication information.

Wang D, Yu B, Li Q, Guo Y, Koike T, Koike Y, Wu Q, Zhang J, Mao L, Tang X, Sun L, Lin X, Wu J, Chen YE, Peng D, Zeng R, lipidome connectivity are associated with atherosclerotic disease. J Mol Cell Biol, 14(3):(2022) [pubmed]

submitter keyword: HDL, high-resolution, proteomics, lipidomics, atherosclerotic disease

Rong Zeng
contact affiliation	Shanghai Institute of Biochemistry and Cell Biology, CAS Center for Excellence in Molecular Cell Science
contact email	zr@sibcb.ac.cn
lab head
Dandan Wang
contact affiliation	Shanghai Institute of Biochemistry and Cell Biology, CAS Center for Excellence in Molecular Cell Science
contact email	wangdandan2015@sibcb.ac.cn
dataset submitter

iProX dataset URI