PXD028209
PXD028209 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | HDL quality feature revealed by proteome-lipidome connectivity are associated with atherosclerotic disease |
Description | Lipoprotein, especially high density lipoprotein (HDL) particles are composed of multiple heterogeneous subgroups containing various proteins and lipids. The molecular distribution among these subgroups is closely related to cardiovascular disease (CVD). Here, we established high-resolution proteomics and lipidomics (HiPL) methods to depict the molecular profiles across lipoprotein (Lipo-HiPL) and HDL (HDL-HiPL) subgroups by optimizing the resolution of anion-exchange chromatography (AEC) and comprehensive quantification of proteins and lipids on omics level. Furthermore, based on the Pearson correlation coefficient (PCC) analysis of molecular profiles across high-resolution subgroups, we achieved the relationship of proteome-lipidome connectivity (PLC) for lipoprotein and HDL particles. By application of these methods to high fat high cholesterol (HFHC) diet rabbits and acute coronary syndrome (ACS) patients, we uncovered the delicate dynamics of molecular profile and reconstruction of lipoprotein and HDL particles. Of note, the PLC features revealed by the HDL-HiPL method discriminated ACS from healthy individuals better than direct proteome and lipidome quantification, or PLC features revealed by the Lipo-HiPL method, suggesting their potential in ACS diagnosis. Together, we established HiPL methods to trace the dynamics of molecular profile and PLC of lipoprotein and even HDL during the development of CVD. |
HostingRepository | iProX |
AnnounceDate | 2021-09-03 |
AnnouncementXML | Submission_2023-02-02_19:08:50.578.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dandan Wang |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Oryctolagus cuniculus; NCBI TaxID: 9986; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-09-05 19:50:08 | ID requested | |
1 | 2021-11-29 19:44:21 | announced | |
⏵ 2 | 2023-02-02 19:08:51 | announced | 2023-02-03: Update publication information. |
Publication List
Wang D, Yu B, Li Q, Guo Y, Koike T, Koike Y, Wu Q, Zhang J, Mao L, Tang X, Sun L, Lin X, Wu J, Chen YE, Peng D, Zeng R, lipidome connectivity are associated with atherosclerotic disease. J Mol Cell Biol, 14(3):(2022) [pubmed] |
Keyword List
submitter keyword: HDL, high-resolution, proteomics, lipidomics, atherosclerotic disease |
Contact List
Rong Zeng | |
---|---|
contact affiliation | Shanghai Institute of Biochemistry and Cell Biology, CAS Center for Excellence in Molecular Cell Science |
contact email | zr@sibcb.ac.cn |
lab head | |
Dandan Wang | |
contact affiliation | Shanghai Institute of Biochemistry and Cell Biology, CAS Center for Excellence in Molecular Cell Science |
contact email | wangdandan2015@sibcb.ac.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |