PXD028202

PXD028202 is an original dataset announced via ProteomeXchange.

Title	Identification of age-associated proteins and functional alterations in human primary retinal pigment epithelium cells
Description	Retinal pigmented epithelium (RPE) has essential functions to nourish and support the neural retina, and is of vital importance in the pathogenesis of age-related retinal degeneration. However, the exact molecular changes of RPE in aging remain poorly defined. We isolated human primary RPE (hRPE) cells from 18 eye donors distributed over a wide age range (10 - 67 years). A quantitative proteomic analysis was performed to analyze their intracellular and secreted protein changes. Age-stage related subtypes and age-associtated proteins were revealed and potential age-associtated mechanisms were validated by ARPE-19 and hRPE cells. Proteomic data and verifications showed that RNF123 and RNF149 related ubiquitin-mediated proteolysis might be an important clearance mechanism in elimination of oxidative damaged proteins in aged hRPE. In older hRPE cells, apoptotic signaling related pathways were up-regulated and endoplasmic reticulum organization was down-regulated both in intracellular and secreted proteome. Our work paints a detailed molecular picture of human RPE in aging process and provides new insights for molecular characteristics of RPE in aging and related clinical retinal conditions.
HostingRepository	iProX
AnnounceDate	2021-09-02
AnnouncementXML	Submission_2021-09-03_03:06:09.902.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xiuxiu jin
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-03 03:05:18	ID requested
⏵ 1	2021-09-03 03:06:10	announced

Dataset with its publication pending

submitter keyword: human primary retinal pigment epithelium cells, aging, proteomics, secreted proteins, ubiquitination

Bo Lei
contact affiliation	Henan Provincial People’s Hospital, Henan Eye Hospital, Henan Eye Institute, People's Hospital of Zhengzhou University, Zhengzhou, Henan, 450003, China
contact email	bolei99@126.com
lab head
xiuxiu jin
contact affiliation	Henan Provincial People’s Hospital, Henan Eye Hospital, Henan Eye Institute, People's Hospital of Zhengzhou University, Zhengzhou, Henan, 450003, China.
contact email	jindaxiu@163.com
dataset submitter

iProX dataset URI