PXD028185

PXD028185 is an original dataset announced via ProteomeXchange.

Title	Mitochondrial inorganic polyphosphate (polyP) is a contributor towards maintaining cellular bioenergetics in SH-SY5Y cells: a proteomics and metabolomics approach
Description	Inorganic polyphosphate (polyP) is an ancestral, ubiquitous, and well-conserved polymer, which is present in all the studied organisms. It is formed by individual subunits of orthophosphate which are linked together by bonds structurally similar and isoenergetic to those found in ATP. While the metabolism and the physiological roles of polyP in some organisms, including bacteria and yeast, have already been described, the exact role of this polymer in mammalian physiology remains still poorly explored. In these organisms, polyP shows a co-localization with mitochondria. Accordingly, its role as a key regulator of bioenergetics has already been demonstrated by our group and others. Here, using Wild-type (Wt) and MitoPPX (cells enzymatically depleted of mitochondrial polyP) SH-SY5Y cells, we conducted a comprehensive study of the status of cellular physiology, using proteomics and metabolomics approaches. Our results suggested a clear dysregulation of mitochondrial physiology, especially of bioenergetics, in MitoPPX cells when compared with Wt. Interestingly, the effects induced by the enzymatically depletion of polyP are similar to those present in the mitochondrial dysfunction which is observed in neurodegenerative disorders and in neuronal aging. In fact, the role of polyP as a component of the cellular stress responses has already been proposed in diverse organisms, including mammals. Thereafter, our data could contribute to place the metabolism of mitochondrial polyP as a valid and innovative pharmacological target in these conditions.
HostingRepository	PanoramaPublic
AnnounceDate	2022-03-16
AnnouncementXML	Submission_2022-03-16_12:12:05.508.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gennifer Merrihew
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-31 22:25:17	ID requested
1	2022-01-05 14:55:42	announced
⏵ 2	2022-03-16 12:12:06	announced	: Added publication

Guitart-Mampel M, Urquiza P, Carnevale Neto F, Anderson JR, Hambardikar V, Scoma ER, Merrihew GE, Wang L, MacCoss MJ, Raftery D, Peffers MJ, Solesio ME, Mitochondrial Inorganic Polyphosphate (polyP) Is a Potent Regulator of Mammalian Bioenergetics in SH-SY5Y Cells: A Proteomics and Metabolomics Study. Front Cell Dev Biol, 10():833127(2022) [pubmed]

submitter keyword: mitochondria, mitochondrial bioenergetics, OXPHOS, inorganic polyphosphate, polyP, metabolomics, proteomics, data-independent acquisiton

Michael MacCoss
contact affiliation	University of Washington
contact email	maccoss@uw.edu
lab head
Gennifer Merrihew
contact affiliation	University of Washington
contact email	genn@uw.edu
dataset submitter

Panorama Public dataset URI