PXD028182

PXD028182 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of saliva of Haemaphysalis longicornis during half blood
Description	Tick saliva, an essential chemical secretion of the tick salivary gland, is indispensable for tick survival owing to the physiological influence it exerts on the host defense mechanisms via the instrumentality of its cocktail of pharmacologically active molecules (proteins and peptides).Here, we performed a phosphoproteomic analysis of Haemaphysalis longicornis tick saliva via LC-MS/MS. A total of 262 phosphorylated tick saliva proteins were identified, and were subjected to functional annotation/enrichment analysis. Cellular and metabolic process terms accounted for the largest proportion of the saliva proteins, with the participation of these proteins in vital intracellular and extracellular transport-oriented processes such as vesicle-mediated transport, exocytic process, cell adhesion, and movement of cell or subcellular component. “Endocytosis”, “Protein processing in endoplasmic reticulum”, and “Purine metabolism” were the most significantly enriched pathways. The knockdown (RNAi) of Tudor domain-containing protein (TCP), actin depolymerizing factors (ADF), programmed cell death protein (PD), and serine/threonine-protein kinase (SPK) resulted in the dissociation of collagen fibers and pilosebaceous unit, increased inflammatory infiltrates/granulocytes (possibly heterophyles), and the depletion of the epithelium.
HostingRepository	iProX
AnnounceDate	2021-12-14
AnnouncementXML	Submission_2022-10-21_02:40:13.783.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ningmei Wang
SpeciesList	scientific name: Haemaphysalis longicornis; NCBI TaxID: 44386;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-31 02:38:05	ID requested
1	2021-08-31 02:40:03	announced
⏵ 2	2022-10-21 02:40:14	announced	2022-10-21: Update publication information.

Agwunobi DO, Wang N, Huang L, Zhang Y, Chang G, Wang K, Li M, Wang H, Liu J, Saliva Reveals the Influential Contributions of Phosphoproteins to Blood-Feeding Success. Front Cell Infect Microbiol, 11():769026(2021) [pubmed]

submitter keyword: Haemaphysalis longicornis,saliva

Hui Wang
contact affiliation	Hebei Normal University
contact email	whui1981@163.com
lab head
Ningmei Wang
contact affiliation	Hebei Normal University
contact email	767247571@qq.com
dataset submitter

iProX dataset URI