PXD028172

PXD028172 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of plasma-derived exosomes extracted by thrombin pre-treated ExoQuick isolation
Description	Exosomes are very small nanosized, membrane-bound vesicles, ranging from 30 to 150 nm in size, are originated and released from a variety of cells from body fluids including blood plasma. The isolation of pure exosome from blood plasma is challenging since it is rich in various contaminants from diverse origins. We applied ExoQuick with thrombin for purification of plasma-derived exosome from healthy individual. Immunoassays confirmed the presence of proteins that are classically associated to exosomes (CD9, CD63 and CD81) but these proteins have not been detected by MS. We found several common proteins enriched in our data set compared with plasma-derived exosome markers from databases. Moreover, proteomics analysis and external database integration revealed an enrichment of those extracellular vesicles. The Gene Ontology data analyses determined the presence of proteins that are involved in catalytic, transporter functions and protein metabolism activities. These results allowed the detection of tetraspanin i.e. TSPAN14 in plasma where proteins were undetected by other studies. Furthermore, this study confirmed the detection of CD5L and LGALS3BP proteins. Although the undouble presence of plasma contaminants hamper MS analysis, still proteins with biomarker potential (TSPAN14, CD5L and LGALS3BP) are accessible in a reasonable sample processing frame that outperforms high-purity focused extraction methods e.g. SEC. The study suggests the use of TSPAN14, CD5L and LGALS3BP as new exosomal markers of plasma-derived exosomes in quantitative proteomics analysis.
HostingRepository	PRIDE
AnnounceDate	2022-03-04
AnnouncementXML	Submission_2022-03-04_05:13:37.112.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028172
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Johannes Schmidt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-27 06:27:45	ID requested
⏵ 1	2022-03-04 05:13:37	announced

Dataset with its publication pending

submitter keyword: Human, plasma-derived exosomes, ExoQuick

Stefan Kalkhof
contact affiliation	Department Preclinical Development and Validation, Unit Proteomics, Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany
contact email	stefan.kalkhof@izi.fraunhofer.de
lab head
Johannes Schmidt
contact affiliation	Fraunhofer Institute for Cell Therapy and Immunology
contact email	johannes.schmidt@izi.fraunhofer.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028172
     1. Label: PRIDE project
     2. Name: Proteomics analysis of plasma-derived exosomes extracted by thrombin pre-treated ExoQuick isolation