PXD028171

PXD028171 is an original dataset announced via ProteomeXchange.

Title	Dynamic interactions of RNA binding proteins to human cytokine mRNA govern T cell responses
Description	Effective T cell responses against infections and tumors depend on the production of effector molecules, including the key pro-inflammatory cytokines IFN-γ, TNF-α and IL-2. Recent studies revealed that post-transcriptional events determine the magnitude and duration of cytokine production in T cells, a feature that is largely defined by RNA-binding proteins (RBPs). However, to date the interplay of RBPs with cytokine mRNA, and their mode of action are ill-defined. Here we employed an RNA-aptamer-based capture assay from human T cell lysates to map RBP interactions with the full length 3’untranslated regions of IFNG, TNF and IL2. We found that RBPs binding can be both promiscuous and cytokine-specific. Furthermore, the RBP binding landscape rapidly alters upon T cell activation. Genetic deletion of confirmed mRNA interactors uncovered RBP-specific activity in primary T cells in response to target cells. Thus, RBPs are critical determinants of fast but tightly controlled cytokine production in T cells.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:01:10.981.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Floris van Alphen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-27 06:27:42	ID requested
1	2023-04-20 05:20:41	announced
⏵ 2	2023-11-14 09:01:11	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: RNA-binding proteins

Prof.Dr. A.B. Meijer
contact affiliation	Department of Molecular and Cellular Hemostasis, Sanquin Research, Amsterdam, The Netherlands
contact email	A.Meijer@sanquin.nl
lab head
Floris van Alphen
contact affiliation	Sanquin Research
contact email	F.vanalphen@sanquin.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD028171

PRIDE project URI

• PRIDE
  1. PXD028171
     1. Label: PRIDE project
     2. Name: Dynamic interactions of RNA binding proteins to human cytokine mRNA govern T cell responses