PXD028163
PXD028163 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Bacterial Progestin and AdipoQ Receptor (PAQR) homologs universally control membrane potential homeostasis |
| Description | Homologs of the Progestin and AdipoQ Receptors (PAQR) are conserved in Bacteria and Eukarya. In eukaryotes, PAQRs are proposed to modulate membrane fluidity and fatty acid (FA) metabolism. The role of bacterial homologs has not yet been elucidated. Here, we show that the gene for PAQR in Escherichia coli is in the unsaturated FA biosynthesis regulon. Lack of bacterial PAQR homologs from the phylogenetically distant E. coli and Bacillus subtilis provoke subtle changes in FA profiles that do not translate to control of membrane fluidity. Instead, using physiological assays and membrane proteomics, we show that these PAQR mutants share a dysregulated membrane potential, which in E. coli, causes increased antibiotic resistance, altered pH homeostasis, and reduced motility and ATP production. The role of PAQR homologs in E. coli and B. subtilis, and thus the shared ancestral role of bacterial PAQRs, is regulation of transmembrane homeostasis linked to FA metabolism. |
| HostingRepository | MassIVE |
| AnnounceDate | 2022-09-28 |
| AnnouncementXML | Submission_2022-09-28_05:46:19.694.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Paul Abraham |
| SpeciesList | scientific name: Escherichia coli; common name: E. coli; NCBI TaxID: 562; scientific name: Bacillus subtilis; NCBI TaxID: 1423; |
| ModificationList | Carbamidomethyl; Oxidation |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-08-26 08:57:36 | ID requested | |
| ⏵ 1 | 2022-09-28 05:46:20 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: proteomics, bacterial membrane |
Contact List
| Gladys Alexandre | |
|---|---|
| contact affiliation | University of Tennessee, Knoxville |
| contact email | galexan2@utk.edu |
| lab head | |
| Paul Abraham | |
| contact affiliation | ORNL |
| contact email | pav@ornl.gov |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000088025/ |
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