PXD028154

PXD028154 is an original dataset announced via ProteomeXchange.

Title	Proteome Analysis of AAV producing HEK293 cells
Description	Adeno-associated viral (AAV) vectors are widely used for gene therapy, providing treatment for diseases caused by absent or defective genes. Despite the success of gene therapy, AAV-manufacturing is still challenging, with production yields being limited. With increased patient demand, improvements in host cell productivity through various engineering strategies will be necessary. Here, we study the host cell proteome of AAV5 producing HEK293 cells using reversed phase nano liquid chromatography and tandem mass spectrometry (LC-MS/MS). Rela-tive label-free quantitation (LFQ) was performed allowing a comparison of transfected vs. un-transfected cells. Gene ontology enrichment and pathway analysis revealed differential expres-sion of proteins involved in fundamental cellular processes such as metabolism, proliferation and cell death. Furthermore, changes in expression of proteins involved in endocytosis and lysosomal degradation were observed. Our data provides highly valuable insights into cellular mechanisms involved during recombinant AAV production by HEK293 cells thus potentially enabling further improvements of gene therapy product manufacturing.
HostingRepository	PRIDE
AnnounceDate	2021-12-09
AnnouncementXML	Submission_2021-12-09_09:01:32.821.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lisa Strasser
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-26 04:07:02	ID requested
⏵ 1	2021-12-09 09:01:33	announced

Strasser L, Boi S, Guapo F, Donohue N, Barron N, Rainbow-Fletcher A, Bones J, Proteomic Landscape of Adeno-Associated Virus (AAV)-Producing HEK293 Cells. Int J Mol Sci, 22(21):(2021) [pubmed]

submitter keyword: HEK293, AAV, LC-MS/MS, proteomics, gene therapy, label free

Jonathan Bones
contact affiliation	NIBRT – National Institute for Bioprocessing Research and Training, Foster Avenue, Blackrock, Dublin, A94 X099, Ireland
contact email	jonathan.bones@nibrt.ie
lab head
Lisa Strasser
contact affiliation	National Institute for Bioprocessing Research and Training, NIBRT
contact email	lisa.strasser@nibrt.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD028154

PRIDE project URI

• PRIDE
  1. PXD028154
     1. Label: PRIDE project
     2. Name: Proteome Analysis of AAV producing HEK293 cells