PXD028140

PXD028140 is an original dataset announced via ProteomeXchange.

Title	RabA2b overexpression alters the Plasma-Membrane Proteome and Improves Drought Tolerance in Arabidopsis
Description	Rab proteins are small GTPases that are important in the regulation of vesicle trafficking. RabA2b was identified through data mining to be stress responsive. Little is known about the involvement of RabA in plant responses to abiotic stresses. Promoter analysis of RabA2b showed strong activity during osmotic stress, which required ABA and was restricted to the vasculature. Sequence analysis of the promoter region identified predicted binding motifs for several ABA-responsive transcription factors. We cloned RabA2b and overexpressed it in Arabidopsis. The resulting transgenic plants were strikingly drought resistant. Subcellular localization studies detected strong colocalization between RabA2b and the plasma membrane (PM) marker PIP2. Further proteomic analysis of isolated PM fractions showed enrichment of stress-coping proteins as well as cell wall/cuticle modifiers in the transgenic lines. Finally, the cuticle permeability of transgenic leaves was significantly reduced compared to the wild type. Overall, these data provide new insights into the roles and modes of action of RabA2b during water stresses, and indicate that increased RabA2b mediated PM trafficking can affect the PM proteome and increase drought tolerance.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_09:04:46.989.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tamar Ziv
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-26 04:05:34	ID requested
⏵ 1	2022-02-15 09:04:47	announced

Ambastha V, Matityahu I, Tidhar D, Leshem Y, RabA2b Overexpression Alters the Plasma-Membrane Proteome and Improves Drought Tolerance in Arabidopsis. Front Plant Sci, 12():738694(2021) [pubmed]

submitter keyword: Plasma-Membrane, Arabidopsis, Drought

Yehoram Leshem
contact affiliation	Departemnt of Plant Sciences MIGAL – Galilee Research Institute, 2 Tarshish st. P.O.B. 831 Kiryat-Shmona 11016 Israel
contact email	yoril@migal.org.il
lab head
Tamar Ziv
contact affiliation	Technion
contact email	tamarz@technion.ac.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028140
     1. Label: PRIDE project
     2. Name: RabA2b overexpression alters the Plasma-Membrane Proteome and Improves Drought Tolerance in Arabidopsis