PXD028121

PXD028121 is an original dataset announced via ProteomeXchange.

Title	Anti-carbamylated fibrinogen antibodies, that might be related to a particular rheumatoid phenotype, include a subset recognizing in vivo epitopes of its γ chain whose one of them is not cross reactive with ACPA
Description	To identify the targets recognized by anti-carbamylated protein (anti-CarP) antibodies in patients with early Rheumatoid Arthritis (RA), to study the cross-reactivity between anti-CarP and anti-citrullinated protein antibodies (ACPA) and to evaluate their prognostic value. 331 patients (184 RA and 147 other rheumatisms) from the Very Early Arthritis (VErA) French cohort were analyzed. We performed mass spectrometry analysis of RA sera displaying anti-CarP activity and epitope mapping of the carbamylated fibrinogen γ chain to identify immunodominant peptides. The specificity of these targets was studied using competition assays with the major antigens recognized by ACPA. The prognostic value of anti-carbamylated fibrinogen IgG antibodies (ACa-Fib IgG) was compared to anti-CCP and anti-CarP using an in-house ELISA. Besides the α chain, the γ chain of fibrinogen, particularly one immunodominant epitope that has a specific reactivity, was identified as a circulating carbamylated target in sera. Prevalence of ACa-Fib was 37% at baseline and 10.9% for anti-CCP-negative RA. In anti-CCP-negative patients, ACa-Fib positivity was associated with a more inflammatory and erosive disease at baseline but not with rapid radiological progression, which remains strongly related to anti-CCP antibodies. Fibrinogen seems to be one of the antigens recognized in vivo by the anti-CarP response, particularly 2 epitopes of the γ chain, one of which is not cross reactive with ACPA. This specificity might be associated with a distinct clinical phenotype since ACa-Fib IgG were shown to be linked to systemic inflammation in very early RA but not to rapid radiological progression.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_04:46:14.686.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Clement GUILLOU
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-25 03:55:44	ID requested
⏵ 1	2021-11-03 04:46:15	announced

Dataset with its publication pending

submitter keyword: Very early Rheumatoid Arthritis, anti-carbamylated protein/peptide antibodies (anti-CarP), fibrinogen, ACPA, prognosis

FRERET Manuel
contact affiliation	Rouen University Hospital, Department of Rheumatology & CIC-CRB1404, Rouen, France. Normandie University, UNIROUEN, INSERM, U1234, Rouen, France
contact email	manuel.freret@inserm.fr
lab head
Clement GUILLOU
contact affiliation	Normandie Université, PISSARO Proteomic Facility, Institute for Research and Innovation in Biomedicine (IRIB), F-76821 Mont-Saint-Aignan, France.
contact email	guillouclement@orange.Fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028121
     1. Label: PRIDE project
     2. Name: Anti-carbamylated fibrinogen antibodies, that might be related to a particular rheumatoid phenotype, include a subset recognizing in vivo epitopes of its γ chain whose one of them is not cross reactive with ACPA