PXD028120

PXD028120 is an original dataset announced via ProteomeXchange.

Title	O-glycoproteome of Porphyromonas gingivalis
Description	Porphyromonas gingivalis is an important human pathogen and also a model organism for the Bacteroidetes phylum. O-glycosylation has been reported in this phylum with findings that include the O-glycosylation motif, the structure of the O-glycans in a few species and an extensive O-glycoproteome analysis in Tannerella forsythia. However, O-glycosylation has not yet been confirmed in P. gingivalis. We therefore used glycoproteomics approaches including partial deglycosylation with trifluoromethanesulfonic acid as well as both HILIC and FAIMS based glycopeptide enrichment strategies leading to the identification of 257 putative glycosylation sites in 145 glycoproteins. The sequence of the major O-glycan was elucidated to be Hex-(dHex)-HexA-Hex-dHex-HexNAc-(P-Gro-[Ac]0-2)-HexNAc. Western blot analyses of mutants lacking the glycosyltransferases PGN_1134 and PGN_1135 demonstrated their involvement in the biosynthesis of the glycan while mass spectrometry analysis of the truncated O-glycans suggested that PGN_1134 and PGN_1135 transfer the two HexNAc sugars. Interestingly, a strong bias against the O-glycosylation of abundant proteins exposed to the cell surface such as abundant T9SS cargo proteins, surface lipoproteins and outer membrane β-barrel proteins was observed. In contrast, the great majority of proteins associated with the inner membrane or periplasm were glycosylated irrespective of their abundance. The P. gingivalis O-glycosylation system may therefore function to establish the desired physicochemical properties of the periplasm.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_09:10:04.926.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028120
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Paul Veith
SpeciesList	scientific name: Porphyromonas gingivalis ATCC 33277; NCBI TaxID: 431947;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-25 03:55:33	ID requested
⏵ 1	2022-02-15 09:10:05	announced

Veith PD, Shoji M, Scott NE, Reynolds EC, Characterization of the O-Glycoproteome of Porphyromonas gingivalis. Microbiol Spectr, 10(1):e0150221(2022) [pubmed]

submitter keyword: Porphyromonas gingivalis, O-glycosylation, Trifluoromethanesulfonic acid, glycan biosynthesis

Eric C Reynolds
contact affiliation	Melbourne Dental School, Bio21 Institute, University of Melbourne
contact email	e.reynolds@unimelb.edu.au
lab head
Paul Veith
contact affiliation	Melbourne Dental School
contact email	pdv@unimelb.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028120
     1. Label: PRIDE project
     2. Name: O-glycoproteome of Porphyromonas gingivalis