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PXD028104

PXD028104 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTranscription Factor Response Elements on Tip: A Sensitive Approach for Large-Scale Endogenous Transcription Factor Quantitative Identification
DescriptionThe ability to map endogenous transcription factors (TFs) DNA binding activity at the proteome scale will greatly enhance our understanding of various biological processes. Here we report a highly sensitive, rapid, and high-throughput approach, transcription factor response elements on tip-mass spectrometry (TOT-MS), that allows for quantitative measurement of endogenous TFs. A total of 150 TFs from 1 μg of nuclear extracts can be quantified with single shot mass spectrometry detection in 1 h of machine time. Up to 755 TFs, which is comparable to the depth of RNA-seq, were identified by TOT coupled with on-tip small size reverse-phase liquid chromatography. We further demonstrated the capability of TOT-MS by interrogating the dynamic change of TFs in the epidermal growth factor (EGF) signaling pathway. This approach should find broad applications in elucidating the TF landscape from limited amounts of biological materials.
HostingRepositoryiProX
AnnounceDate2021-08-25
AnnouncementXMLSubmission_2021-08-25_01:07:56.140.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWenhao Shi
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-08-25 01:06:47ID requested
12021-08-25 01:07:56announced
Publication List
Shi W, Li K, Song L, Liu M, Wang Y, Liu W, Xia X, Qin Z, Zhen B, Wang Y, He F, Qin J, Ding C, Transcription Factor Response Elements on Tip: A Sensitive Approach for Large-Scale Endogenous Transcription Factor Quantitative Identification. Anal Chem, 88(24):11990-11994(2016) [pubmed]
Keyword List
submitter keyword: Transcription factors,TOT,EGF
Contact List
Chen Ding
contact affiliationBeijing Proteome Research Center
contact emailchend@fudan.edu.cn
lab head
Wenhao Shi
contact affiliationBeijing Proteome Research Center
contact emailwhaosmith@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI