PXD028086

PXD028086 is an original dataset announced via ProteomeXchange.

Title	Determination of allergen levels, isoforms and their hydroxyproline modifications among peanut genotypes by mass spectrometry
Description	The recently published genome of peanut enables detailed molecular description of seed storage proteins, particularly allergens, which make up the peanut seed proteome. A detailed description of the allergenic proteome was produced using peanuts of different genetic backgrounds to increase the coverage of potentially expressed proteins and additionally allows for analysis of protein variability. Using relative quantification by mass spectrometry, minor variation of some allergenic proteins was observed but total levels of Ara h 1, 2, 3 and 6 were relatively consistent among genotypes. The isoform compositions of Ara h 1, 2 and 6 were similar. Three Ara h 3 isoforms were variable among genotypes and contributed to a large proportion of total Ara h 3 when present. The immunologically relevant amino acid modification hydroxyproline, previously thought to be present only on Ara h 2, was detected on Ara h 1 and 3 in consistent proportions given when the associated isoforms were present. The relevance of the data was evaluated in terms of food allergy regarding applications to reference materials, diagnostic and immunotherapeutic use, detection of peanuts in food, and risk assessment. The data can be used to design targeted mass spectrometry methods to permit analytical control of such materials.
HostingRepository	PRIDE
AnnounceDate	2022-08-11
AnnouncementXML	Submission_2022-08-11_05:22:30.800.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028086
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Justin Marsh
SpeciesList	scientific name: Arachis hypogaea (Peanut); NCBI TaxID: 3818;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-24 03:07:39	ID requested
⏵ 1	2022-08-11 05:22:33	announced

Marsh JT, Palmer LK, Koppelman SJ, Johnson PE, Determination of Allergen Levels, Isoforms, and Their Hydroxyproline Modifications Among Peanut Genotypes by Mass Spectrometry. Front Allergy, 3():872714(2022) [pubmed]

submitter keyword: Arachis hypogaea, Food Allergy, Hydroxyproline, Mass spectrometry, Peanut

Philip Johnson
contact affiliation	Assistant Professor Department of Food Science & Technology Food Allergy Research & Resource Program University of Nebraska-Lincoln
contact email	philip.johnson@unl.edu
lab head
Justin Marsh
contact affiliation	University of Nebraska-Lincoln
contact email	Justin.marsh@unl.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028086
     1. Label: PRIDE project
     2. Name: Determination of allergen levels, isoforms and their hydroxyproline modifications among peanut genotypes by mass spectrometry