PXD028078

PXD028078 is an original dataset announced via ProteomeXchange.

Title	Paediatric Saliva Protein Library (Burn patients and Healthy Controls) and SWATH analysis
Description	A paediatric saliva protein spectral library was created using drool samples collected from children with small area burn injury as well as healthy controls. Iso-electric focussing, LDS-PAGE and a combination of both fractionation methods were utilised to increase the protein coverage of the library. This resulted in the identification of 1310 proteins at a 10% FDR cut-off. The library samples were analysed using DDA-MS. The generated protein spectral library was used to quantify the abundance of 742 proteins in each individual participant sample using SWATH-MS. All samples were run on a Sciex TripleTOF 6600 mass spectrometer, and the data was processed using the OneOmics Suite v3.0 (SCIEX). Index retention time proteins were used in this experiment. The effect of burn injury and various burn characteristics on salivary protein abundance profiles were evaluated.
HostingRepository	PRIDE
AnnounceDate	2025-01-29
AnnouncementXML	Submission_2025-01-29_01:32:27.660.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leila Cuttle
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-24 02:30:58	ID requested
⏵ 1	2025-01-29 01:32:28	announced

10.1002/PRCA.202300107;

submitter keyword: Human, burns, paediatric, saliva

Leila Cuttle
contact affiliation	Centre for Children’s Burn and Trauma Research, Centre for Children’s Health Research, Queensland University of Technology, South Brisbane, Queensland, Australia
contact email	leila.cuttle@qut.edu.au
lab head
Leila Cuttle
contact affiliation	Queensland University of Technology
contact email	leila.cuttle@qut.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD028078

PRIDE project URI

• PRIDE
  1. PXD028078
     1. Label: PRIDE project
     2. Name: Paediatric Saliva Protein Library (Burn patients and Healthy Controls) and SWATH analysis