PXD028072

PXD028072 is an original dataset announced via ProteomeXchange.

Title	Identification and function of components from a divergent mRNA export pathway in trypanosomes
Description	mRNA export is central to gene regulation and expression. In trypanosomes, transcription is polycistronic, all mRNAs are processed by trans-splicing and export is mediated by non-canonical mechanisms. We have described several conserved mRNA export pathway components in Trypanosoma cruzi, including orthologs of Sub2, a component of the TREX complex, and eIF4AIII, a core component of the exon junction complex (EJC). Few orthologs of proteins involved in mRNA export in higher eukaryotes are detectable in trypanosome genomes and examples of mechanistic divergence well known. To uncover additional components of the trypanosome mRNA maturation and export system we undertook an unbiased search for protein interactors of TcSub2 and TceIF4AIII. Significant overlap between TcSub2 and TceIF4AIII interacting protein cohorts suggests that both proteins associate with similar machinery. We confirmed several interactions with conserved core components of the EJC and multiple additional complexes, together with identification of proteins specific to trypanosomatids. The highly interactive super-interactome uncovered here, capable of supporting RNA processing from splicing through to nuclear export and cytoplasmic events, highlights kinetoplastid-specific and conserved components creating an amalgam to support the unique mRNA maturation mechanisms in trypanosomes.
HostingRepository	PRIDE
AnnounceDate	2022-02-07
AnnouncementXML	Submission_2022-02-07_08:21:54.491.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Patricia Domingues
SpeciesList	scientific name: Trypanosoma cruzi Dm28c; NCBI TaxID: 1416333;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL ETD

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-23 07:49:26	ID requested
1	2022-02-07 08:16:27	announced
⏵ 2	2022-02-07 08:21:55	announced	2022-02-07: Updated publication reference for PubMed record(s): 35091090.

Inoue AH, Domingues PF, Serpeloni M, Hiraiwa PM, Vidal NM, Butterfield ER, Del Pino RC, Ludwig A, Boehm C, Field MC, Á, vila AR, Proteomics Uncovers Novel Components of an Interactive Protein Network Supporting RNA Export in Trypanosomes. Mol Cell Proteomics, 21(3):100208(2022) [pubmed]

submitter keyword: mRNA export, mRNA processing, EJC, TREX complex, trypanosomes, gene expression, proteomics, evolution, LC-MS/MS

Andréa Rodrigues Ávila
contact affiliation	Laboratório de Pesquisa em Apicomplexa - LAPAPI, Instituto Carlos Chagas - Fiocruz Paraná
contact email	andrea.avila@fiocruz.br
lab head
Patricia Domingues
contact affiliation	Carlos Chagas Institute
contact email	pfdominguess@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028072
     1. Label: PRIDE project
     2. Name: Identification and function of components from a divergent mRNA export pathway in trypanosomes