PXD028061

PXD028061 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic analysis identifies supervillin as a new ERK3 substrate regulating cytokinesis and ploidy of breast cancer cells
Description	Extracellular signal-regulated kinase 3 (ERK3) is a poorly characterized member of the mitogen-activated protein (MAP) kinase family. Functional analysis of the ERK3 signaling pathway has been hampered by a lack of knowledge about the substrates and downstream effectors of the kinase. Here, we used large-scale quantitative phosphoproteomics and targeted gene silencing to identify direct ERK3 substrates and gain insight into its potential cellular functions. Detailed validation of one candidate substrate identified the gelsolin/villin family member supervillin (SVIL) as a bona fide ERK3 substrate. We show that ERK3 phosphorylates SVIL on Ser245 to regulate myosin II activation and cytokinetic furrowing in dividing cells. Depletion of SVIL or ERK3 leads to cytokinesis failure and multinucleation, a phenotype rescued by wild type SVIL but not by the non-phosphorylatable S245A mutant. Our results unveil a new function of the atypical MAP kinase ERK3 in cell division and the regulation of cell ploidy
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:33:34.218.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eric Bonneil
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-23 06:36:13	ID requested
1	2023-02-21 10:37:08	announced
⏵ 2	2023-11-14 07:33:35	announced	2023-11-14: Updated project metadata.

Javary J, Goupil E, Soulez M, Kanshin E, Bouchard A, Seternes OM, Thibault P, Labb, é JC, Meloche S, Phosphoproteomic analysis identifies supervillin as an ERK3 substrate regulating cytokinesis and cell ploidy. J Cell Physiol, 239(3):e30938(2024) [pubmed]

submitter keyword: breast cancer, cytokinesis, supervillin, ERK3, ploidy,Phosphoprotoemic

Sylvain Meloche
contact affiliation	IRIC-Université de Montréal
contact email	sylvain.meloche@umontreal.ca
lab head
Eric Bonneil
contact affiliation	Proteomic Platform
contact email	eric.bonneil@umontreal.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD028061

PRIDE project URI

• PRIDE
  1. PXD028061
     1. Label: PRIDE project
     2. Name: Phosphoproteomic analysis identifies supervillin as a new ERK3 substrate regulating cytokinesis and ploidy of breast cancer cells