PXD028051

PXD028051 is an original dataset announced via ProteomeXchange.

Title	Specific deletion of protein phosphatase 6 catalytic subunit in Sertoli cells leads to disruption of spermatogenesis
Description	Protein phosphatase 6 (PP6) is a member of the PP2A-like subfamily, which plays significant roles in numerous fundamental biological activities. We found that PPP6C plays important roles in male germ cells recently. Spermatogenesis is supported by the Sertoli cells in seminiferous epithelium. In this study, we crossed Ppp6cF/F mice with AMH-Cre mice to gain mutant mice with specific depletion of the Ppp6c gene in the Sertoli cells. We discovered that the PPP6C cKO male mice were absolutely infertile and germ cells were largely lost during spermatogenesis. By combing phosphoproteome with bioinformatics analysis, we showed that the phosphorylation status of β-catenin at S552 (a marker of adherens junctions) was significantly upregulated in mutant mice. Abnormal β-catenin accumulation resulted in impaired testicular junction integrity, thus led to abnormal structure and functions of BTB. Taken together, our study reveals a novel function for PPP6C in male germ cell survival and differentiation by regulating the cell-cell communication through dephosphorylating β-catenin at S552.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_08:00:39.695.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenlong Lei
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-20 07:34:13	ID requested
⏵ 1	2022-02-15 08:00:40	announced

Lei WL, Li YY, Meng TG, Ning Y, Sun SM, Zhang CH, Gui Y, Wang ZB, Qian WP, Sun QY, Specific deletion of protein phosphatase 6 catalytic subunit in Sertoli cells leads to disruption of spermatogenesis. Cell Death Dis, 12(10):883(2021) [pubmed]

submitter keyword: PPP6C, spermatogenesis, phosphoproteome, Sertoli cells, β-catenin

Wen-Long Lei
contact affiliation	Department of Reproductive Medicine, Peking University Shenzhen Hospital, Shenzhen 518036, China
contact email	wenlonglei@163.com
lab head
Wenlong Lei
contact affiliation	State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences
contact email	wenlonglei@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028051
     1. Label: PRIDE project
     2. Name: Specific deletion of protein phosphatase 6 catalytic subunit in Sertoli cells leads to disruption of spermatogenesis