PXD028048

PXD028048 is an original dataset announced via ProteomeXchange.

Title	Structural characterization of the reaction center-light harvesting 1-PufXY complex from Rhodobacter sphaeroides
Description	Photosynthesis in purple bacteria, represented by Rhodobacter sphaeroides as a long-established model organism, utilizes a protein-cofactor complex to capture solar energy. This complex is embedded in specialized spherical membrane structures and comprises a central reaction center (RC) encircled by a ring of light harvesting 1 (LH1) polypeptides. The energy derived from light absorption drives the reduction of ubiquinone, entering from the membrane, to generate ubiquinol. The photosynthetic cycle is therefore dependent on the exchange of ubiquinone and ubiquinol to and from the RC. This exchange requires an additional protein (PufX) which prevents complete closure of the LH1 ring, leaving a channel which connects the RC with the membrane. The structure of the RC-LH1-PufX complex is under investigation by cryo-EM, revealing the presence of a previously undiscovered protein (PufY) that is also involved in channel formation. Proteomic analysis has verified the identities of the expected protein components of this complex, additionally confirming the presence of the newly-discovered PufY polypeptide mapped into the cryo-EM structure.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_02:32:35.274.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philip Jackson
SpeciesList	scientific name: Rhodobacter sphaeroides 2.4.1; NCBI TaxID: 272943;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-20 06:08:02	ID requested
⏵ 1	2022-02-15 02:32:36	announced

Qian P, Swainsbury DJK, Croll TI, Salisbury JH, Martin EC, Jackson PJ, Hitchcock A, Castro-Hartmann P, Sader K, Hunter CN, . Biochem J, 478(20):3775-3790(2021) [pubmed]

submitter keyword: LC-MS/MS, Rhodobacter sphaeroides, purple bacteria, photosynthesis, reaction center light-harvesting-1-PufXY complex

Christopher Neil Hunter
contact affiliation	Department of Molecular Biology and Biotechnology, University of Sheffield, UK
contact email	c.n.hunter@sheffield.ac.uk
lab head
Philip Jackson
contact affiliation	Department of Molecular Biology and Biotechnology, University of Sheffield
contact email	p.j.jackson@sheffield.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD028048

PRIDE project URI

• PRIDE
  1. PXD028048
     1. Label: PRIDE project
     2. Name: Structural characterization of the reaction center-light harvesting 1-PufXY complex from Rhodobacter sphaeroides