PXD028047

PXD028047 is an original dataset announced via ProteomeXchange.

Title	The periplasmic oxidoreductase DsbA is required for virulence of the phytopathogen Dickeya solani
Description	The DsbA oxidoreductase is a crucial factor responsible for introduction of disulfide bonds to the extracytoplasmic proteins in bacteria. A lack of the proper disulfides frequently leads to instability and/or loss of protein function. In pathogens, numerous envelope and extracellular proteins play important roles in pathogenesis; therefore, their improper disulfide bonding may lead to avirulent phenotypes. The importance of the DsbA function in phytopathogens has not been extensively studied yet. Dickeya solani is a bacterium from the Soft Rot Pectobacteriaceae group which is responsible for very high economic losses mainly on potato. In recent years, D. solani became the most abundant potato pathogen among Dickeya species in Europe. In this work, using the D. solani dsbA mutant, we demonstrated that a lack of the DsbA function caused loss of virulence. Mutant bacteria were deficient in most secreted virulence determinants and were not able to develop disease symptoms in the natural host, the potato plant. The SWATH-MS-based proteomic analysis revealed that the dbsA mutation led to multifaceted effects in the D. solani cells. First of all, the levels of the majority of plant cell wall degrading enzymes and proteins related to motility and chemotaxis were severely reduced. Furthermore, the protein profiles suggested induction of the envelope and cytoplasm stress responses in the mutant cells. Finally, the outer membrane barrier seemed to be disturbed by the mutation. Our results clearly demonstrate that the function played by the DsbA oxidoreductase is indispensable for D. solani virulence and a lack of DsbA significantly disturbs cellular physiology. A thorough analysis of proteomic research suggests that a lack of virulence may result from both, abnormalities of the disulfide deprived virulence determinants and the envelope stress-dependent repression of the virulence genes in the dsbA mutant.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_02:38:35.330.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aleksandra Lewandowska
SpeciesList	scientific name: Dickeya solani; NCBI TaxID: 1089444;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600+

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-20 06:08:01	ID requested
⏵ 1	2022-02-15 02:38:36	announced

Przepiora T, Figaj D, Bogucka A, Fikowicz-Krosko J, Czajkowski R, Hugouvieux-Cotte-Pattat N, Skorko-Glonek J, . Int J Mol Sci, 23(2):(2022) [pubmed]

submitter keyword: Dickeya solani, DsbA, LC-MS/MS, SWATH-MS

Paulina Czaplewska
contact affiliation	Laboratory of Mass Spectrometry, Core Facility Laboratories, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdansk, Gdansk, Poland
contact email	paulina.czaplewska@ug.edu.pl
lab head
Aleksandra Lewandowska
contact affiliation	University of Gdansk
contact email	aleksandra.lewandowska@biotech.ug.edu.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028047
     1. Label: PRIDE project
     2. Name: The periplasmic oxidoreductase DsbA is required for virulence of the phytopathogen Dickeya solani