PXD028034

PXD028034 is an original dataset announced via ProteomeXchange.

Title	Interactome of POU5F1B detected by LC-MSMS in HT29 and LS1034 CRC cells overexpressing POU5F1B-HA
Description	The treatment of colorectal cancer (CRC) is an unmet medical need in absence of early diagnosis. Here, upon characterizing cancer-specific transposable element-driven transpochimeric gene transcripts (TcGTs) produced by this tumor, we found that expression of the hominid-restricted retrogene POU5F1B through aberrant activation of a primate-specific endogenous retroviral promoter is a strong negative prognostic CRC biomarker. Correlating this observation, we could demonstrate that POU5F1B fosters the proliferation and metastatic potential of CRC cells. We further determined that POU5F1B, in spite of its phylogenetic relationship with the POU5F1/OCT4 transcription factor, is a membrane-enriched protein that associates with protein kinases and known targets or interactors as well as with cytoskeleton-related molecules, and induces intracellular signaling events and the release of trans-acting factors involved in cell growth and cell adhesion. As POU5F1B is an apparently non-essential gene only lowly expressed in normal tissues, and as POU5F1B-containing TcGTs are detected in other tumors besides CRC, our data provide interesting leads for the development of cancer therapies.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:32:04.771.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julien Duc
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-19 21:35:15	ID requested
1	2022-10-14 09:20:14	announced
⏵ 2	2023-11-14 08:32:10	announced	2023-11-14: Updated project metadata.

Sim, ó, -Riudalbas L, Offner S, Planet E, Duc J, Abrami L, Dind S, Coudray A, Coto-Llerena M, Ercan C, Piscuoglio S, Andersen CL, Bramsen JB, Trono D, Transposon-activated POU5F1B promotes colorectal cancer growth and metastasis. Nat Commun, 13(1):4913(2022) [pubmed]

submitter keyword: Human, LC-MSMS, POU5F1B, HT29, LS1034, overexpression, colorectal cancer

Didier Trono
contact affiliation	EPFL
contact email	didier.trono@epfl.ch
lab head
Julien Duc
contact affiliation	EPFL
contact email	julien.duc@epfl.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD028034

PRIDE project URI

• PRIDE
  1. PXD028034
     1. Label: PRIDE project
     2. Name: Interactome of POU5F1B detected by LC-MSMS in HT29 and LS1034 CRC cells overexpressing POU5F1B-HA