PXD028031
PXD028031 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Interactomic analysis reveals a new homeostatic role for the HIV restriction factor TRIM5 alpha in mitophagy |
| Description | The protein TRIM5 alpha has multiple roles in anti-retroviral defense, but the mechanisms underlying TRIM5 alpha action are unclear. Here, we used an APEX2-based proteomics approach to identify TRIM5 alpha-interacting proteins. Analysis of the TRIM5 alpha interactome found proteins participating in a wide variety of cellular functions including regulating antiviral signaling pathways. We used this data set to uncover a novel role for TRIM5 alpha in mitophagy, an autophagy-based mode of mitochondrial quality control that is compromised in multiple human diseases. Mitochondrial damage triggered the relocalization of TRIM5 alpha to ER-mitochondria contact sites where TRIM5 alpha colocalized with markers of autophagy initiation and autophagosome biogenesis. Furthermore, we found that TRIM5 alpha knockout attenuated both Parkin-dependent and Parkin-independent mitophagy by preventing the recruitment of autophagy regulators FIP200 and ATG13 to unhealthy mitochondria. Finally, TRIM5 alpha knockout cells showed reduced mitochondrial function under basal conditions and were more susceptible to uncontrolled immune activation and cell death in response to mitochondrial damage than were wild type cells. Taken together, our studies have identified a homeostatic role for a protein previously recognized exclusively for its antiviral actions. |
| HostingRepository | MassIVE |
| AnnounceDate | 2021-08-20 |
| AnnouncementXML | Submission_2021-08-20_17:42:07.046.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Brett Phinney |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Oxidation; Carbamidomethyl |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-08-19 17:26:06 | ID requested | |
| ⏵ 1 | 2021-08-20 17:42:07 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: TRIM5a |
Contact List
| Michael A Mandell | |
|---|---|
| contact affiliation | Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, |
| contact email | mmandell@salud.unm.edu |
| lab head | |
| Brett Phinney | |
| contact affiliation | UC Davis |
| contact email | brettsp@ucdavis.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000088006/ |
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