PXD028027 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparative Proteomic Analysis to Reveal Key Maize Low Nitrogen Responsive Genes |
Description | Two maize hybrid cultivars contrasting in low nitrogen tolerance (low nitrogen-tolerant XY335 and low nitrogen-sensitive HN178) were used in this study . The experiment was carried out at Xinji Experimental Station (43º31′N, 124º48′E) of Hebei Agricultural University. The top 0-20 cm of the soil used contained organic matter 17.79 g·kg-1, total nitrogen 1.21 g·kg-1, alkali hydrolyzed nitrogen 64.9 mg.kg-1, available phosphorus 23.8 mg·kg-1, and available potassium 120.6 mg·kg-1. The experiment adopted a split plot design, with varieties as the main plot and nitrogen fertilizer as the sub-plot. There were 2 varieties for testing: XY335 and HN138. Two levels of nitrogen supply: N0 (0 kg N ha-1) and N240 (240 kg N ha-1), replicated three times. Each plot had 6 rows, with the row length measuring 20 m, and the row spacing of 60 cm, giving the plot area of 72 m2. The planting density was 67,500 plants ha-1. Nitrogen fertilizer used was urea (46% N), and 50% was applied before sowing and at the flared stage, respectively. During the grain filling stage, leaf tissues of three biological replicates were collected from control and treatment conditions, and immediately frozen in liquid nitrogen for subsequent proteomics analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-20 |
AnnouncementXML | Submission_2022-05-20_03:58:37.158.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jiao Li |
SpeciesList | scientific name: Zea mays (Maize); NCBI TaxID: 4577; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-08-19 01:42:10 | ID requested | |
⏵ 1 | 2022-05-20 03:58:38 | announced | |
Publication List
Wang Y, Wang N, Liu S, Dong A, Zenda T, Liu X, Li J, Duan H, Gene. Genes (Basel), 13(4):(2022) [pubmed] |
Keyword List
submitter keyword: maize,leaf,low nitrogen |
Contact List
Huijun Duan |
contact affiliation | College of Agronomy, Hebei Agricultural University, Baoding, China |
contact email | hjduan@hebau.edu.cn |
lab head | |
Jiao Li |
contact affiliation | Hebei Agricultural University, Baoding City, Hebei Province, China |
contact email | LJ337871790219@163.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028027
- Label: PRIDE project
- Name: Comparative Proteomic Analysis to Reveal Key Maize Low Nitrogen Responsive Genes