PXD028014

PXD028014 is an original dataset announced via ProteomeXchange.

Title	Differential protein expression profiles of testes in Tmprss12-/- and wild-type mice
Description	Serine protease is considered a relative upstream regulator in the physiological processes including male reproduction. Transmembrane serine protease 12 (TMPRSS12) has been shown to regulate sperm motility and uterotubal junction migration in mice, but its role in the testis remains unknown.In this study, we confirmed the important role of TMPRSS12 in spermatogenesis. To further investigate the mechanism of TMPRSS12 in male reproduction, we used 2-D electrophoresis combined with Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry(MALDI-TOF/TOF) to construct differential protein expression profiles of testes in Tmprss12-/- and WT mice. A total of 64 spots with 2-fold or more differential expression between two groups were identified using MALDI-TOF/TOF. As a result, 54 unique proteins were identified successfully, including 39 proteins with decreased expression and 15 proteins with increased expression in Tmprss12-/- mice.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-20_03:42:00.228.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinli Zhou
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	ultraflex

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-18 08:50:17	ID requested
⏵ 1	2022-05-20 03:42:01	announced

Zhang J, Zhou X, Wan D, Yu L, Chen X, Yan T, Wu Z, Zheng M, Zhu F, Zhu H, TMPRSS12 Functions in Meiosis and Spermiogenesis and Is Required for Male Fertility in Mice. Front Cell Dev Biol, 10():757042(2022) [pubmed]

submitter keyword: TMPRSS12, serine protease, testis

Hui Zhu
contact affiliation	State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, China
contact email	njzhuhui@njmu.edu.cn
lab head
Xinli Zhou
contact affiliation	Nanjing Medical University
contact email	xzxznsyz@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD028014

PRIDE project URI

• PRIDE
  1. PXD028014
     1. Label: PRIDE project
     2. Name: Differential protein expression profiles of testes in Tmprss12-/- and wild-type mice