PXD028008

PXD028008 is an original dataset announced via ProteomeXchange.

Title	Evaluation of affinity purified RNA-protein complexes related with NMD
Description	Nonsense-mediated mRNA decay (NMD) is a conserved RNA degradation pathway that is involved in development, resistance to viral infections and evolution. Upf1, a core NMD factor, is associated with a large variety of cellular RNA in complex ribonucleoprotein particles. We characterized NMD complexes previously by affinity purification of tagged protein followed by mass spectrometry. Here, we extend our observations to proteins that are present in NMD complexes, but for whom the interaction with NMD factors is mediated by RNA. We purified tagged versions of Lsm1, Lsm7, Pat1, Dhh1 and Pab1 and assessed the presence and amount of NMD and RNA-related proteins in each purification. As expected from our previously published results, Upf1 was present in these purifications, suggesting novel hypotheses about the role of Pab1 and the Lsm1-7 complexes in RNA degradation.
HostingRepository	PRIDE
AnnounceDate	2023-08-30
AnnouncementXML	Submission_2023-08-30_01:34:48.864.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028008
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	CosminSaveanu
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-18 05:40:27	ID requested
⏵ 1	2023-08-30 01:34:49	announced

10.6019/PXD028008;

submitter keyword: Saccharomyces cerevisiae, RNA, RNP, Upf1,yeast

CosminSaveanu
contact affiliation	Génétique des Interactions Macromoléculaires, Genomes and Genetics Department Institut Pasteur, 25-28 rue du docteur Roux, 75015, Paris, France
contact email	cosmin.saveanu@pasteur.fr
lab head
CosminSaveanu
contact affiliation	Institut Pasteur, Paris, France
contact email	cosmin.saveanu@pasteur.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028008
     1. Label: PRIDE project
     2. Name: Evaluation of affinity purified RNA-protein complexes related with NMD