PXD028005

PXD028005 is an original dataset announced via ProteomeXchange.

Title	Conformation of the AP-3 complex explains its role in cargo recruitment at the Golgi
Description	Crosslinking mass spectrometry study of an important adapter protein complex at the Golgi AP-3. We performed crosslinking mass spectrometry study using crosslinker DSSO and analyzed the digested peptides by LC-MS. The crosslinking study provided important information the subunit connectivity of AP-3, which facilitated the structural analysis of AP-3 by cyro-EM.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_06:17:38.222.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Heike Stephanowitz
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-18 04:13:07	ID requested
⏵ 1	2022-02-17 06:17:39	announced

Schoppe J, Schubert E, Apelbaum A, Yavavli E, Birkholz O, Stephanowitz H, Han Y, Perz A, Hofnagel O, Liu F, Piehler J, Raunser S, Ungermann C, Flexible open conformation of the AP-3 complex explains its role in cargo recruitment at the Golgi. J Biol Chem, 297(5):101334(2021) [pubmed]

submitter keyword: AP-3, crosslinking, mass spectrometry, structure

Christian Ungermann
contact affiliation	University of Osnabrück, Department of Biology/Chemistry, Biochemistysection, Osnabrück, Germany
contact email	Christian.Ungermann@biologie.uni-osnabrueck.de
lab head
Heike Stephanowitz
contact affiliation	Leibniz-Forschungsinstitut für Molekulare Pharmakologie
contact email	stephanowitz@fmp-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD028005

PRIDE project URI

• PRIDE
  1. PXD028005
     1. Label: PRIDE project
     2. Name: Conformation of the AP-3 complex explains its role in cargo recruitment at the Golgi