PXD027998

PXD027998 is an original dataset announced via ProteomeXchange.

Title	Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans
Description	Using C. elegans germ granule, called P granule, as a model system, we employed a proximity-based labeling method in combination with mass spectrometry to comprehensively define its protein components. This set of experiments identified over 200 proteins, many of which contain intrinsically disordered regions. An RNAi-based screen identified factors that are essential for P granule assembly, notably EGGD-1 and EGGD-2, two previously uncharacterized LOTUS-domain proteins. Loss of eggd-1 and eggd-2 results in separation of P granules from nuclear envelope, germline atrophy and reduced fertility. We show that intrinsically disordered regions of EGGD-1 are required to anchor EGGD-1 to the nuclear periphery while its LOTUS domains are required to promote perinuclear localization of P granules. Together, our work expands the repertoire of P granule constituents and provides new insights into the role of LOTUS-domain proteins in germ granule organization.
HostingRepository	PRIDE
AnnounceDate	2021-11-12
AnnouncementXML	Submission_2021-11-12_04:23:02.413.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	WEN TANG
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-18 00:06:23	ID requested
⏵ 1	2021-11-12 04:23:02	announced

Dataset with its publication pending

submitter keyword: germ granules, Proximity labeling, C. elegans

Wen Tang
contact affiliation	Ohio State University
contact email	tang.542@osu.edu
lab head
WEN TANG
contact affiliation	Ohio State University
contact email	tang.542@osu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027998
     1. Label: PRIDE project
     2. Name: Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans