PXD027992 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Nanodiscs-based proteomics to identify lipid-interacting proteins in yeast |
Description | Many soluble proteins interact with membranes to perform important biological functions, including signal transduction, regulation, transport, trafficking and biogenesis. Despite their importance, these protein-membrane interactions are difficult to characterize due to their often-transient nature as well as phospholipids’ poor solubility in aqueous solution. Here, we employ nanodiscs – small, water-soluble patches of lipid bilayer encircled with amphipathic scaffold proteins – along with quantitative proteomics to identify lipid-binding proteins in S. cerevisiae. Using nanodiscs reconstituted with yeast total lipid extracts or only phosphatidylethanolamine (PE-nanodiscs), we capture several known membrane-interacting proteins, including the Rab GTPases Sec4 and Ypt1, which play key roles in vesicle trafficking. Utilizing PE-nanodiscs enriched with phosphatidic acid (PEPA-nanodiscs), we specifically capture a member of the Hsp40/J-protein family, Caj1, whose function has recently been linked to membrane protein quality control. We show that Caj1 interaction with liposomes containing PA is modulated by pH and PE lipids, and depends on two patches of positively charged residues near the C-terminus of the protein. The protein Caj1 is the first example of an Hsp40/J-domain protein with affinity for membranes and phosphatidic acid lipid specificity. These findings highlight the utility of the nanodisc system to identify and characterize protein-lipid interactions that may not be evident using other methods. |
HostingRepository | PRIDE |
AnnounceDate | 2021-12-30 |
AnnouncementXML | Submission_2021-12-30_14:08:52.265.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | John Young |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-08-17 23:07:12 | ID requested | |
⏵ 1 | 2021-12-30 14:08:52 | announced | |
Publication List
Zhang XX, Young JW, Foster LJ, Duong F, Nanodisc-Based Proteomics Identify Caj1 as an Hsp40 with Affinity for Phosphatidic Acid Lipids. J Proteome Res, 20(10):4831-4839(2021) [pubmed] |
Keyword List
submitter keyword: Nanodiscs, SILAC labeling, Hsp40, Caj1, Rab GTPase |
Contact List
Franck Duong |
contact affiliation | Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver BC, Canada |
contact email | fduong@mail.ubc.ca |
lab head | |
John Young |
contact affiliation | University of Oxford |
contact email | john.young@chem.ox.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027992
- Label: PRIDE project
- Name: Nanodiscs-based proteomics to identify lipid-interacting proteins in yeast