PXD027985

PXD027985 is an original dataset announced via ProteomeXchange.

Title	A comparative study of synthetic winged peptides for absolute protein quantification
Description	A proper internal standard choice is critical for accurate, precise, and reproducible mass spectrometry-based proteomics assays. Synthetic isotopically labeled (SIL) proteins are currently considered the gold standard. However, they are costly and challenging to obtain. An alternative approach uses SIL peptides or SIL "winged" peptides extended at C- or/and N-terminus with an amino acid sequence or a tag cleaved during enzymatic proteolysis. However, a consensus on the design of a winged peptide for absolute quantification is missing. In this study, we used human serum albumin as a model system to compare the quantitative performance of reference SIL protein with four different designs of SIL winged peptides: i) commercially available SIL peptides with a proprietary trypsin cleavable tag at C-terminus, ii) SIL peptides extended with five amino acid residues at C-terminus, iii) SIL peptides extended with three and iv) with five amino acid residues at both C- and N-termini. Our results demonstrate properties of various SIL extended peptides designs, e.g., water solubility and efficiency of trypsin enzymatic cleavage with primary influence on quantitative performance. SIL winged peptides extended with three amino acids at both C- and N- termini demonstrated optimal quantitative performance, equivalent to the SIL protein.
HostingRepository	PanoramaPublic
AnnounceDate	2021-08-17
AnnouncementXML	Submission_2021-08-17_20:43:34.929.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Zdenek Spacil
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	6495B Triple Quadrupole LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-17 12:40:49	ID requested
⏵ 1	2021-08-17 20:43:35	announced

Benesova E, Vidova V, Spacil Z, A comparative study of synthetic winged peptides for absolute protein quantification. Sci Rep, 11(1):10880(2021) [pubmed]

submitter keyword: targeted proteomics assays, internal standards, SIL-proteins, SIL-peptides, synthetic winged peptides, absolute protein quantification

Zdenek Spacil
contact affiliation	Masaryk University, Faculty of Science, RECETOX
contact email	spacil@recetox.muni.cz
lab head
Zdenek Spacil
contact affiliation	Masaryk University, Faculty of Science, RECETOX
contact email	spacil@recetox.muni.cz
dataset submitter

Panorama Public dataset URI