PXD027972

PXD027972 is an original dataset announced via ProteomeXchange.

Title	Proteomic Profiling Identifies Novel Diagnostic Biomarkers and Molecular subtypes for Mucinous Tubular and Spindle Cell Carcinoma of the Kidney
Description	Mucinous tubular and spindle cell carcinoma (MTSCC) is a relatively rare renal epithelial neoplasm resembling type 1 papillary renal cell carcinoma (PRCC) morphologically and immunohistochemically. The accurate diagnosis of MTSCC remains a challenge. Here, by using proteomic profiling, we characterized MTSCC and PRCC to identify diagnostic biomarkers. We found that MTSCC tumor proteome was significantly enriched in B cell-mediated immunity when compared with the proteome of adjacent normal tissues of MTSCC or tumors of PRCC. Importantly, we identified MZB1, VCAN, and SOSTDC1 as diagnostic biomarkers to distinguish MTSCC from PRCC, with an AUC of 0.9839 when combined. MZB1 was inversely correlated with tumor clinical stage and may play an anti-tumor role by activating the complement system. Finally, unsupervised clustering revealed two molecular subtypes of MTSCC, displaying different morphology, expression signatures of oxidative phosphorylation, and aggravation. In summary, our analyses identified a three-protein diagnostic panel and molecular subtypes for MTSCC.
HostingRepository	PRIDE
AnnounceDate	2022-02-11
AnnouncementXML	Submission_2022-02-11_07:08:17.316.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fen Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-17 04:30:53	ID requested
1	2022-02-11 06:55:16	announced
⏵ 2	2022-02-11 07:08:18	announced	2022-02-11: Updated publication reference for PubMed record(s): 35043389.

Xu H, Li W, Zhu C, Cheng N, Li X, Hao F, Zhu J, Huang L, Wang R, Wang L, Luo Z, Wang F, Proteomic profiling identifies novel diagnostic biomarkers and molecular subtypes for mucinous tubular and spindle cell carcinoma of the kidney. J Pathol, 257(1):53-67(2022) [pubmed]

submitter keyword: Kidney, mucinous tubular and spindle cell carcinoma, proteomic, immunohistochemistry

Fen Wang
contact affiliation	The First Affiliated Hospital, Sun Yat-Sen University
contact email	wangfen5@mail.sysu.edu.cn
lab head
Fen Wang
contact affiliation	The First Affiliated Hospital, Sun Yat-Sen University
contact email	wangfen5@mail.sysu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD027972

PRIDE project URI

• PRIDE
  1. PXD027972
     1. Label: PRIDE project
     2. Name: Proteomic Profiling Identifies Novel Diagnostic Biomarkers and Molecular subtypes for Mucinous Tubular and Spindle Cell Carcinoma of the Kidney