PXD027961

PXD027961 is an original dataset announced via ProteomeXchange.

Title	tipNrich, a tip-based N-terminal proteome enrichment method
Description	Protein N-termini provide valuable information such as half-life of protein, but one of hurdles to study N-terminome is that it requires large amount of sample, but this requirement might be problematic for analyzing the limited samples such as low number of cells of clinical biopsies. In this regard, we developed tip-based N-terminome enrichment method, tipNrich. Our method, the scale-down version of our previously introduced N-terminome enrichment method iNrich, provides simple and fast N-terminone analysis platform even for analyzing small amount of protein. Our method could be utilized either for quality check of protein products or as a diagnostic tool for human diseases related to N-terminal proteoforms. We applied our method to various recombinant single proteins, protein mixture, and plasma samples of wild-type transthyretin amyloidosis (WTTA) patients.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:19:35.518.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Seonjeong Lee
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Bacillus subtilis; NCBI TaxID: 1423; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ; Q Exactive; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-17 04:04:52	ID requested
1	2022-12-05 02:20:50	announced
⏵ 2	2023-11-14 08:19:38	announced	2023-11-14: Updated project metadata.

Lee S, Ju S, Kim SJ, Choi JO, Kim K, Kim D, Jeon ES, Lee C, tipNrich: A Tip-Based N-Terminal Proteome Enrichment Method. Anal Chem, 93(42):14088-14098(2021) [pubmed]

submitter keyword: Human plasma,Recombinant single proteins, Protein mixture, LC-MS/MS

Cheolju Lee
contact affiliation	Center for Theragnosis, Korea Institute of Science and Technology, Seoul, Korea
contact email	clee270@kist.re.kr
lab head
Seonjeong Lee
contact affiliation	Korea Institute of Science and Technology
contact email	sejelee@kist.re.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD027961

PRIDE project URI

• PRIDE
  1. PXD027961
     1. Label: PRIDE project
     2. Name: tipNrich, a tip-based N-terminal proteome enrichment method