PXD027960

PXD027960 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	One-step Isolation of Protein C-terminal Peptides from Protease-digested Proteins by Metal Oxide-based Ligand Exchange Chromatography
Description	We have developed a one-step method to isolate protein C-terminal peptides from V8-digested proteins by metal oxide-based ligand-exchange (MOLEX) chromatography. V8 protease cleaves the C-terminal side of Asp and Glu, and the resulting digested peptide has two carboxy groups at the C-terminus, whereas the protein C-terminal peptide has only one α-carboxy group. In MOLEX chromatography, a stable chelate is formed between dicarboxylates and metal atoms, so that the non-terminal (i.e., internal) peptide is retained, but the protein C-terminal peptide flows through the MOLEX column. By optimizing the conditions of V8 protease digestion and MOLEX chromatographic isolation, a total of 1619 protein C-termini from 30 μg of peptides derived from human HeLa cells could be identified in 10 min of the isolation step. Furthermore, using 200 µg of peptide, we identified 2202 protein C-termini, which was the largest data set with the least amount of sample in protein C-terminomics. These results indicated that this method is unprecedentedly simple and sensitive for protein C-terminal peptide enrichment.
HostingRepository	jPOST
AnnounceDate	2021-12-28
AnnouncementXML	Submission_2022-09-18_03:07:59.602.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hiroshi Nishida
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	TripleTOF 5600; Q Exactive; instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-17 00:52:01	ID requested
1	2021-12-27 19:55:32	announced
2	2022-01-04 21:06:20	announced	2022-01-05: Updated PubMed.
⏵ 3	2022-09-18 03:08:00	announced	2022-09-18: Updated FTP location.

Publication List

Nishida H, Ishihama Y, One-Step Isolation of Protein C-Terminal Peptides from V8 Protease-Digested Proteins by Metal Oxide-Based Ligand-Exchange Chromatography. Anal Chem, 94(2):944-951(2022) [pubmed]

Nishida H, Ishihama Y, One-Step Isolation of Protein C-Terminal Peptides from V8 Protease-Digested Proteins by Metal Oxide-Based Ligand-Exchange Chromatography. Anal Chem, 94(2):944-951(2022) [pubmed]

Keyword List

submitter keyword: Protein C-terminal peptide enrichment, metal oxide ligand exchange chromatography, C-terminomics

submitter keyword: Protein C-terminal peptide enrichment, metal oxide ligand exchange chromatography, C-terminomics

Contact List

Yasushi Ishihama
lab head
Hiroshi Nishida
contact affiliation	Kyoto university
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001284/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001284/