PXD027955

PXD027955 is an original dataset announced via ProteomeXchange.

Title	Hypoxia-reoxygenation stress response in the marine facultative anaerobe Mytilus edulis
Description	Hypoxia is a characteristic feature of marine environments and a major stressor for marine organisms inhabiting benthic and intertidal zones. Several studies have explored the responses of these organisms to hypoxic stress at the whole organism level with a focus on energy metabolism and mitochondrial response, but the instrinsic mitochondrial responses that support the organelle’s function under hypoxia and reoxygenation (H/R) stress are not well understood. We studied the effects of acute H/R stress (10 min anoxia followed by 15 min reoxygenation) on mitochondrial respiration, production of reactive oxygen species (ROS) and posttranslational modifications (PTM) of the proteome in a marine facultative anaerobe, the blue mussel Mytilus edulis. The mussels’ mitochondria showed increased OXPHOS respiration and suppressed proton leak resulting in a higher coupling efficiency after H/R stress. ROS production decreased in both the resting (LEAK) and phosphorylating (OXPHOS) state indicating that M. edulis is able to prevent oxidative stress and mitochondrial damage during reoxygenation. Hypoxia did not stimulate the rearrangement of the mitochondrial supercomplexes but impacted the mitochondrial phosphoproteome including the proteins involved in OXPHOS, amino acid and fatty acid catabolism, and protein quality control. This study indicates that mussels’ mitochondria possess intrinsic mechanisms (including regulation via PTM mechanisms such as reversible protein phosphorylation) that ensure high respiratory flux and mitigate oxidative damage during H/R stress and contribute to the hypoxia-tolerant mitochondrial phenotype of this metabolically plastic species.
HostingRepository	PRIDE
AnnounceDate	2021-11-25
AnnouncementXML	Submission_2021-11-25_07:26:49.150.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027955
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Stephanie Markert
SpeciesList	scientific name: Mytilus edulis; NCBI TaxID: 6550;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-16 06:20:01	ID requested
⏵ 1	2021-11-25 07:26:49	announced

Dataset with its publication pending

submitter keyword: Phosphoproteins, mussels, mitochondria, supercomplexes, hypoxia-reoxygenation, taurine

Stephanie Markert
contact affiliation	Universität Greifswald Institut für Pharmazie, AG Pharmazeutische Biotechnologie Greifswald, Germany
contact email	stephanie.markert@uni-greifswald.de
lab head
Stephanie Markert
contact affiliation	University of Greifswald
contact email	stephanie.markert@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027955
     1. Label: PRIDE project
     2. Name: Hypoxia-reoxygenation stress response in the marine facultative anaerobe Mytilus edulis