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PXD027944

PXD027944 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAnalysing alpha4 and alpha9 integrin complex partners
DescriptionAdhesion of basal keratinocytes to the underlying extracellular matrix (ECM) plays a key role in the control of skin homeostasis and response to injury. Integrin receptors indirectly link the ECM to the cell cytoskeleton through large protein complexes called focal adhesions (FA). FA also function as intracellular biochemical signaling platforms to enable cells to respond to changing extracellular cues. The α4β1 and α9β1 integrins are both expressed in basal keratinocytes, share some common ECM ligands, and have been shown to promote wound healing in vitro and in vivo. However, their roles in maintaining epidermal homeostasis and relative contributions to pathological processes in the skin remain unclear. We found that α4β1 and α9β1 occupied distinct regions in monolayers of a basal keratinocyte cell line (NEB-1). During collective cell migration (CCM), α4 and α9 integrins co-localized along the leading edge. Pharmacological inhibition of α4β1 and α9β1 integrins increased keratinocyte proliferation and induced a dramatic change in cytoskeletal remodeling and FA rearrangement, detrimentally affecting collective cell migration (CCM). Further analysis revealed that α4β1/α9β1 integrins suppress ERK1/2 activity to control migration through the regulation of downstream kinases including Mitogen and Stress Activated Kinase 1 (MSK1). We performed LC-MS/MS analysis of α4β1/α9β1 adhesion complexes to identify partners that could regulate ERK activity.
HostingRepositoryPRIDE
AnnounceDate2022-02-17
AnnouncementXMLSubmission_2022-02-17_06:14:02.890.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD027944
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterWillow Hight-Warburton
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-08-16 06:00:12ID requested
12022-02-17 06:14:03announced
Publication List
Hight-Warburton W, Felix R, Burton A, Maple H, Chegkazi MS, Steiner RA, McGrath JA, Parsons M, 9 Integrins Coordinate Epithelial Cell Migration Through Local Suppression of MAP Kinase Signaling Pathways. Front Cell Dev Biol, 9():750771(2021) [pubmed]
Keyword List
submitter keyword: Human, keratinocytes, LC-MS/MS
Contact List
Maddy Parsons
contact affiliationParsons Group Randall Centre for Cell & Molecular Biophysics King's College London UK
contact emailmaddy.parsons@kcl.ac.uk
lab head
Willow Hight-Warburton
contact affiliationKing's College London
contact emailwillow.h.hight-warburton@kcl.ac.uk
dataset submitter
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Dataset FTP location
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