PXD027934

PXD027934 is an original dataset announced via ProteomeXchange.

Title	Yorkie and Bonus protein interactome-Hippo pathway and Bonus control developmental cell fate decisions in the Drosophila eye
Description	To identify novel regulators of the Hippo pathway, we performed affinity purification-mass spectrometry (AP-MS) using Drosophila embryos overexpressing Yki-EGFP with a ubiquitous driver da-GAL4, as well as cultured S2 cells expressing Yki-SBP. We identified the core Hippo pathway components, multiple Hippo pathway regulators and functional groups, and several putative Yki interactors including Bonus (Bon). To identify additional cofactors that are recruited by Bon, we performed AP-MS using Bon-SBP expressed in Drosophila S2 cells. Further genetic tests revealed the involvement of Bon interactors, HDAC1, Su(var)2-10, and Hrb27C, in the Drosophila eye specification that is regulated by the Yki-Bon complex.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:44:15.685.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Heya Zhao
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-16 00:38:53	ID requested
1	2023-01-17 10:11:00	announced
2	2023-03-14 07:59:27	announced	2023-03-14: Updated project metadata.
⏵ 3	2023-11-14 08:44:21	announced	2023-11-14: Updated project metadata.

Zhao H, Moberg KH, Veraksa A, Hippo pathway and Bonus control developmental cell fate decisions in the Drosophila eye. Dev Cell, 58(5):416-434.e12(2023) [pubmed]

submitter keyword: embryo,Drosophila, Yki, S2 cell, Bon, LC-MS/MS

Alexey Veraksa
contact affiliation	Biology Department, University of Massachusetts Boston, USA
contact email	alexey.veraksa@umb.edu
lab head
Heya Zhao
contact affiliation	University of Massachusetts Boston
contact email	heya.zhao001@umb.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD027934

PRIDE project URI

• PRIDE
  1. PXD027934
     1. Label: PRIDE project
     2. Name: Yorkie and Bonus protein interactome-Hippo pathway and Bonus control developmental cell fate decisions in the Drosophila eye