PXD027923

PXD027923 is an original dataset announced via ProteomeXchange.

Title	Analysis of the impact of DICER1 and DROSHA on the angio-genic capacity of human endothelial cells
Description	RNAi-mediated knockdown of DICER1 and DROSHA, enzymes critically involved in miRNA biogenesis, has been postulated to affect the homeostasis and the angiogenic capacity of human endothelial cells. To re-evaluate this issue, we reduced the expression of DICER1 or DROSHA by RNAi-mediated knockdown and subsequently investigated the effect of these interventions on the angiogenic capacity of human umbilical vein endothelial cells (HUVEC) in vitro (proliferation, migration, tube formation, endothelial cell spheroid sprouting) and in a HUVEC xenograft assay in immune incompetent NSGTM mice in vivo. In contrast to previous reports, neither knockdown of DICER1 nor knockdown of DROSHA profoundly affected migration or tube formation of HUVEC or the angiogenic capacity of HUVEC in vivo. Furthermore, knockdown of DICER1 and the combined knockdown of DICER1 and DROSHA tended to increase VEGF-induced BrdU incorporation and induced angiogenic sprouting from HUVEC spheroids. Consistent with these observations, global proteomic analyses showed that knockdown of DICER1 or DROSHA only moderately altered HUVEC protein expression profiles but additively reduced, for example, expression of the angiogenesis inhibitor thrombospondin-1. In conclusion, global reduction of miRNA biogenesis by knockdown of DICER1 or DROSHA does not inhibit the angiogenic capacity of HUVEC. Further studies are therefore needed to elucidate the influence of these enzymes in the context of human endothelial cell-related angiogenesis.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_06:17:29.248.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027923
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matt Fuszard
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-13 04:04:59	ID requested
⏵ 1	2022-02-17 06:17:33	announced

Braun H, Hauke M, Ripperger A, Ihling C, Fuszard M, Eckenstaler R, Benndorf RA, Impact of DICER1 and DROSHA on the Angiogenic Capacity of Human Endothelial Cells. Int J Mol Sci, 22(18):(2021) [pubmed]

submitter keyword: Endothelial cells

angiogenesis

microRNA biogenesis, DICER1

DROSHA

RNA interference

Ralf A. Benndorf
contact affiliation	Prof. Dr. Ralf Benndorf Abteilung für Klinische Pharmazie und Pharmakotherapie Institut für Pharmazie Martin-Luther-Universität Halle-Wittenberg
contact email	ralf.benndorf@pharmazie.uni-halle.de
lab head
Matt Fuszard
contact affiliation	Martin-Luther-Üniversität Halle-Wittenberg
contact email	matthew.fuszard@medizin.uni-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027923
     1. Label: PRIDE project
     2. Name: Analysis of the impact of DICER1 and DROSHA on the angio-genic capacity of human endothelial cells