PXD027919

PXD027919 is an original dataset announced via ProteomeXchange.

Title	Cross-species analysis of viral nucleic acid interactors identifies TAOKs as immune regulators
Description	The cell intrinsic antiviral response of multicellular organisms developed over millions of years and critically relies on the ability to sense and eliminate viral nucleic acids. Here we used an affinity proteomics approach in evolutionary distant species (human, mouse and fly) to identify proteins that are conserved in their ability to associate with diverse viral nucleic acids. This approach revealed a conserved core of proteins targeting viral genetic material and species-specific interactants. Functional characterization of the influence of 181 candidates on replication of 6 distinct viruses in human cells and flies identified 128 nucleic acid binding proteins with an impact on virus growth. We identified the family of TAO kinases (TAOK1, -2 and -3) as dsRNA-interacting antiviral proteins and show their requirement in type-I interferon induction. Depletion of TAO kinases in mammals or flies led to an impaired response to virus infection characterized by a reduced induction of interferon stimulated genes in mammals and impaired expression of srg1 and diedel in flies. Overall, our study reveals a by far larger interaction space between viral genetic material and host factors than anticipated so far, attesting to the ancestral roots of innate immunity and to the lineage-specific pressures exerted by viruses.
HostingRepository	PRIDE
AnnounceDate	2023-04-11
AnnouncementXML	Submission_2023-04-11_06:18:10.907.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ChristianUrban
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-13 03:10:47	ID requested
1	2021-09-22 01:19:11	announced
⏵ 2	2023-04-11 06:18:11	announced	2023-04-11: Updated project metadata.

Pennemann FL, Mussabekova A, Urban C, Stukalov A, Andersen LL, Grass V, Lavacca TM, Holze C, Oubraham L, Benamrouche Y, Girardi E, Boulos RE, Hartmann R, Superti-Furga G, Habjan M, Imler JL, Meignin C, Pichlmair A, Cross-species analysis of viral nucleic acid interacting proteins identifies TAOKs as innate immune regulators. Nat Commun, 12(1):7009(2021) [pubmed]

submitter keyword: AP-MS,LC-MS/MS

AndreasPichlmair
contact affiliation	Technical University of Munich, School of Medicine, Institute of Virology, 81675 Munich, Germany
contact email	andreas.pichlmair@tum.de
lab head
ChristianUrban
contact affiliation	Institute of Virology, Technical University Munich
contact email	c.urban@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD027919

PRIDE project URI

• PRIDE
  1. PXD027919
     1. Label: PRIDE project
     2. Name: Cross-species analysis of viral nucleic acid interactors identifies TAOKs as immune regulators