PXD027909

PXD027909 is an original dataset announced via ProteomeXchange.

Title	Identification of protein corona on graphene oxide in B medium
Description	As antimicrobials, graphene materials (GMs) may have advantages over traditional antibiotics due to their physical mechanisms of action which ensure less chance of development of microbial resistance. However, the fundamental question as to whether the antibacterial mechanism of GMs originates from penetration by their sharp edges or wrapping of the cell membrane, or from a combination of these and other mechanisms, remains controversial. In this study, we show both experimentally and theoretically that GMs with high surface oxygen content (SOC) predominantly attach in parallel to the bacterial cell surface when in the suspension phase. The interaction mode shifts to perpendicular interaction when the SOC reaches a threshold of ~ 0.3 (the atomic percent of O in the total atoms). However, neither mode necessarily kills bacteria. Rather, bactericidal activity depends on the interaction of GMs with surrounding biomolecules. Proteomic study shows that protein corona forms on the surface graphene oxide in LB medium, thus explain the different antibacterial activity in different culture medium.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:38.347.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peng Zhang
SpeciesList	scientific name: fungal yeast sp. S26-10; NCBI TaxID: 479096;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-12 15:14:20	ID requested
1	2023-05-10 13:36:17	announced
⏵ 2	2023-11-14 08:46:41	announced	2023-11-14: Updated project metadata.

Guo Z, Zhang P, Xie C, Voyiatzis E, Faserl K, Chetwynd AJ, Monikh FA, Melagraki G, Zhang Z, Peijnenburg WJGM, Afantitis A, Chen C, Lynch I, Defining the Surface Oxygen Threshold That Switches the Interaction Mode of Graphene Oxide with Bacteria. ACS Nano, 17(7):6350-6361(2023) [pubmed]

submitter keyword: graphene oxide, protein corona, LB medium

Peng Zhang
contact affiliation	University of Birmingham
contact email	p.zhang.1@bham.ac.uk
lab head
Peng Zhang
contact affiliation	University of Birmingham
contact email	p.zhang.1@bham.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD027909

PRIDE project URI

• PRIDE
  1. PXD027909
     1. Label: PRIDE project
     2. Name: Identification of protein corona on graphene oxide in B medium