PXD027903

PXD027903 is an original dataset announced via ProteomeXchange.

Title	Identification of polysome-associated translational regulators during oxidative stress and amino acid starvation
Description	To identify RBPs with previously unappreciated roles in translational regulation, we performed polysome proteomics (polysome profiling and label-free mass spectrometry) to identify and quantify proteins associated with translating ribosomes in unstressed yeast cells and changes in association during two acute stresses that induce the integrated stress response (ISR): oxidative stress (0.45 mM H2O2 treatment for 15 min) and amino acid starvation (10 mM 3-AT treatment for 15 min). Five fractions were isolated from sucrose gradients prepared from each extract, corresponding to monosomes (F1) and polysomes with increasing numbers of ribosomes per mRNA (F2-F5). Unfractionated cyotplasmic lysates (totals) were analysed for each sample. Four replicate extracts were prepared and analysed for each condition.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:53:25.876.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robert Crawford
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-12 14:25:24	ID requested
1	2022-06-13 06:33:07	announced
⏵ 2	2023-11-14 08:53:26	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: polysomes, yeast, S. cerevisiae, stress response, ribosome, translation

Professor Graham Pavitt
contact affiliation	Division of Molecular & Cellular Function, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Science Centre, Oxford Road, Manchester, M13 9PT
contact email	graham.pavitt@manchester.ac.uk
lab head
Robert Crawford
contact affiliation	University of Manchester
contact email	rcrawford527@hotmail.co.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD027903

PRIDE project URI

• PRIDE
  1. PXD027903
     1. Label: PRIDE project
     2. Name: Identification of polysome-associated translational regulators during oxidative stress and amino acid starvation