PXD027902

PXD027902 is an original dataset announced via ProteomeXchange.

Title	Proteomics-Based Retinal Target Engagement Analysis and Retina-Targeted Delivery of 17β-Estradiol by the DHED Prodrug in Males. Katalin Prokai-Tatrai,* Khadiza Zaman, Vien Nguyen, Daniel L. De La Cruz and Laszlo Prokai
Description	We examined the impact of 17β-estradiol (E2) eye drops on the modulation of the proteome pro-file in the male rat retina. With discovery-driven proteomics, we have identified proteins that were regulated by the treatment. These proteins were assembled to several bioinformatics-based networks implicating E2’s beneficial effects on the male rat retina in a broad context of ocular neuroprotection including the maintenance of retinal homeostasis, facilitation of efficient dis-posal of damaged proteins, and mitochondrial respiratory chain biogenesis. We have also shown for the first time that the hormone’s beneficial effects on the male retina can be con-strained to this target site by treatment with the bioprecursor prodrug DHED. A large concen-tration of E2 was produced after DHED eye drops not only in male rat retinae but also in those of rabbits. However, DHED treatment did not increase circulating E2 levels thereby ensuring therapeutic safety in males. Targeted proteomics focusing on selected biomarkers of E2’s target engagement further confirmed the prodrug’s metabolism to E2 in the male retina and indicated that the retinal impact of DHED treatment was identical to that of the direct E2 treatment. Alto-gether, our study shows the potential of topical DHED therapy for an efficacious and safe protec-tion of the male retina without unwanted hormonal side-effects associated with current estrogen therapies.
HostingRepository	PRIDE
AnnounceDate	2021-11-04
AnnouncementXML	Submission_2021-11-04_03:27:12.863.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027902
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Laszlo Prokai
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-12 14:24:46	ID requested
⏵ 1	2021-11-04 03:27:13	announced

Prokai-Tatrai K, Zaman K, Nguyen V, De La Cruz DL, Prokai L, -Estradiol by the DHED Prodrug for Ocular Neurotherapy in Males. Pharmaceutics, 13(9):(2021) [pubmed]

submitter keyword: DHED prodrug, 17β-estradiol

E2

eye drops

male retina

parallel-reaction monitoring, prote-omics, neuroprotection

LC-MS/MS, isotope-dilution, targeted proteomics

Laszlo Prokai, Ph.D
contact affiliation	Professor and Robert A. Welch Chair in Biochemistry Department of Pharmacology and Neuroscience University of North Texas Health Science Center, FortWorth
contact email	Laszlo.Prokai@unthsc.edu
lab head
Laszlo Prokai
contact affiliation	Department of Pharmacology & Neuroscience UNT Health Science Center Fort Worth, TX, USA
contact email	advancedms@unthsc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD027902

PRIDE project URI

• PRIDE
  1. PXD027902
     1. Label: PRIDE project
     2. Name: Proteomics-Based Retinal Target Engagement Analysis and Retina-Targeted Delivery of 17β-Estradiol by the DHED Prodrug in Males. Katalin Prokai-Tatrai,* Khadiza Zaman, Vien Nguyen, Daniel L. De La Cruz and Laszlo Prokai