PXD027895

PXD027895 is an original dataset announced via ProteomeXchange.

Title	Matrisome age-related changes in the mouse gastrocnemius muscle
Description	In recent years, thanks to the great development of mass spectrometry (MS)-based high-throughput proteomic techniques, a large-scale protein characterization is less challenging. Proteomic approaches have been successfully applied to SM in different experimental models [21–24]; however, to date, there is little information on ECM proteins and how they change during ageing. The identification and quantification of ECM components and their interactions are es-sential steps to understand the role of the matrisome in sarcopenia. In this context, we combined a proteomic approach (i.e., LC-MS/MS and bioinformatic analyses) with mor-phological and morphometrical evaluations at fluorescence and transmission electron microscopy of the gastrocnemius muscle in adult and old mice. This age range has been selected since it has been observed that most of changes in muscle protein expression take place after middle age. The gastrocnemius muscle was selected for analysis since it is prevalently composed of fast-twitch fibre, which are especially affected by atrophy during aging. Our findings highlighted several modifications of ECM protein composition and organization in old mice, which likely play a role in the alteration of muscle properties during aging.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_06:35:21.220.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francesco Demetrio Lofaro
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-12 06:53:00	ID requested
⏵ 1	2022-02-17 06:35:22	announced

Lofaro FD, Cisterna B, Lacavalla MA, Boschi F, Malatesta M, Quaglino D, Zancanaro C, Boraldi F, Age-Related Changes in the Matrisome of the Mouse Skeletal Muscle. Int J Mol Sci, 22(19):(2021) [pubmed]

submitter keyword: aging

extracellular matrix

proteomics

ultrastructure

skeletal muscle

sarcopenia

Federica, Boraldi
contact affiliation	University of Modena and Reggio Emilia
contact email	federica.boraldi@unimore.it
lab head
Francesco Demetrio Lofaro
contact affiliation	University of Modena and Reggio Emilia
contact email	francescodemetrio.lofaro@unimore.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD027895

PRIDE project URI

• PRIDE
  1. PXD027895
     1. Label: PRIDE project
     2. Name: Matrisome age-related changes in the mouse gastrocnemius muscle