PXD027862

PXD027862 is an original dataset announced via ProteomeXchange.

Title	CSN5i-3 induces degradation of selective CRL substrate receptors and accumulation of CRL substrates
Description	In this study, we used quantitative proteomics mass spectrometry with 16-plex TMT labeling to compare individual protein levels of DMSO and 1 μM CSN5i-3-treated K562 cells for 2, 8, and 24 hours. CSN5i-3 is a selective and potent inhibitor of Cop9 Signalosome (CSN), which regulates the activity of Cullin-RING E3 ubiquitin ligases (CRLs). CSN5i-3 treatment resulted in reduced CSN activity, and consequently increased cullin neddylation and constitutively active CRL. Gene Ontology analysis of the changed proteins between DMSO- and CSN5i-3-treated samples showed the enrichment of CSN subunits, cell cycle and chromosome-related components, and phosphatase complex, which include multiple CSN subunits (e.g., CSN7B and CSN5), components of CRLs, especially CRL SRs (e.g., SKP2, ELOA and DCAF1/VPRBP), and known substrates of CRLs (e.g., MAGEA6, GLUL, and RHOB). Indeed, eight out of the top 20 most decreased proteins were CRL adaptor and substrate receptors, two out of the top 20 were E2 proteins (CDC34/UBE2R1 and UBE2R2), and two were CSN subunits. Eight out of the top 20 most increased proteins were reported CRL substrates.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:53:27.149.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brett Lomenick
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-11 04:27:53	ID requested
1	2022-09-04 21:07:02	announced
⏵ 2	2023-11-14 08:53:28	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: CRL, substrate receptor, mass spectrometry, CSN5i-3,Cop9 signalosome, quantitative proteomics

Spiros D Garbis
contact affiliation	Caltech Proteome Exploration Laboratory
contact email	s.d.garbis@gmail.com
lab head
Brett Lomenick
contact affiliation	Caltech Proteome Exploration Lab
contact email	lomenick@caltech.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD027862

PRIDE project URI

• PRIDE
  1. PXD027862
     1. Label: PRIDE project
     2. Name: CSN5i-3 induces degradation of selective CRL substrate receptors and accumulation of CRL substrates